Cysteine Protease-Binding Protein Family 6 Mediates the Trafficking of Amylases to Phagosomes in the Enteric Protozoan Entamoeba histolytica
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- Atsushi Furukawa
- Department of Parasitology, National Institute of Infectious Diseases, Toyama, Tokyo, Japan
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- Kumiko Nakada-Tsukui
- Department of Parasitology, National Institute of Infectious Diseases, Toyama, Tokyo, Japan
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- Tomoyoshi Nozaki
- Department of Parasitology, National Institute of Infectious Diseases, Toyama, Tokyo, Japan
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- J. F. Urban
- editor
説明
<jats:title>ABSTRACT</jats:title> <jats:p> Phagocytosis plays a pivotal role in nutrient acquisition and evasion from the host defense systems in <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">Entamoeba histolytica</jats:named-content> , the intestinal protozoan parasite that causes amoebiasis. We previously reported that <jats:named-content xmlns:xlink="http://www.w3.org/1999/xlink" content-type="genus-species" xlink:type="simple">E. histolytica</jats:named-content> possesses a unique class of a hydrolase receptor family, designated the cysteine protease-binding protein family (CPBF), that is involved in trafficking of hydrolases to lysosomes and phagosomes, and we have also reported that CPBF1 and CPBF8 bind to cysteine proteases or β-hexosaminidase α-subunit and lysozymes, respectively. In this study, we showed by immunoprecipitation that CPBF6, one of the most highly expressed CPBF proteins, specifically binds to α-amylase and γ-amylase. We also found that CPBF6 is localized in lysosomes, based on immunofluorescence imaging. Immunoblot and proteome analyses of the isolated phagosomes showed that CPBF6 mediates transport of amylases to phagosomes. We also demonstrated that the carboxyl-terminal cytosolic region of CPBF6 is engaged in the regulation of the trafficking of CPBF6 to phagosomes. Our proteome analysis of phagosomes also revealed new potential phagosomal proteins. </jats:p>
収録刊行物
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- Infection and Immunity
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Infection and Immunity 81 (5), 1820-1829, 2013-05
American Society for Microbiology
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詳細情報 詳細情報について
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- CRID
- 1360848660904428544
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- ISSN
- 10985522
- 00199567
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- データソース種別
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- Crossref
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