Non-apoptotic function of <i>Drosophila</i> caspase activation in epithelial thorax closure and wound healing

DOI PDF Web Site 被引用文献5件 参考文献74件 オープンアクセス
  • Yuya Fujisawa
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 1 Department of Genetics ,   Bunkyo -ku   Tokyo 113-0033
  • Hina Kosakamoto
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 1 Department of Genetics ,   Bunkyo -ku   Tokyo 113-0033
  • Takahiro Chihara
    Graduate School of Science, Hiroshima University 2 Department of Biological Science , , Higashi-Hiroshima, Hiroshima 739-8526 , Japan
  • Masayuki Miura
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, 1 Department of Genetics ,   Bunkyo -ku   Tokyo 113-0033

書誌事項

公開日
2019-02-15
資源種別
journal article
権利情報
  • http://www.biologists.com/user-licence-1-1/
DOI
  • 10.1242/dev.169037
公開者
The Company of Biologists

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説明

<jats:title>ABSTRACT</jats:title> <jats:p>Non-apoptotic caspase activation involves multiple cellular events. However, the link between visible non-apoptotic caspase activation and its function in living organisms has not yet been revealed. Here, we visualized sub-lethal activation of apoptotic signaling with the combination of a sensitive indicator for caspase 3 activation and in vivo live-imaging analysis of Drosophila. During thorax closure in pupal development, caspase 3 activation was specifically observed at the leading edge cells, with no signs of apoptosis. Inhibition of caspase activation led to an increase in thorax closing speed, which suggests a role of non-apoptotic caspase activity in cell motility. Importantly, sub-lethal activation of caspase 3 was also observed during wound closure at the fusion sites at which thorax closure had previously taken place. Further genetic analysis revealed that the activation of the initiator caspase Dronc is coupled with the generation of reactive oxygen species. The activation of Dronc also regulates myosin levels and delays wound healing. Our findings suggest a possible function for non-apoptotic caspase activation in the fine-tuning of cell migratory behavior during epithelial closure.</jats:p>

収録刊行物

  • Development

    Development 146 (4), dev160937-, 2019-02-15

    The Company of Biologists

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