<i>In Situ</i>
            Structure of the
            <i>Vibrio</i>
            Polar Flagellum Reveals a Distinct Outer Membrane Complex and Its Specific Interaction with the Stator

Shiwei Zhu, Tatsuro Nishikino, Norihiro Takekawa, Hiroyuki Terashima, Seiji Kojima, Katsumi Imada, Michio Homma, Jun Liu

doi:10.1128/jb.00592-19

<jats:p> The torque of flagellar rotation is generated by interactions between a rotor and a stator; however, detailed structural information is lacking. Here, we utilized cryo-electron tomography and advanced imaging analysis to obtain a high-resolution <jats:italic>in situ</jats:italic> flagellar basal body structure in <jats:named-content content-type="genus-species">Vibrio alginolyticus</jats:named-content> , which is a Gram-negative marine bacterium. Our high-resolution motor structure not only revealed detailed protein-protein interactions among unique <jats:italic>Vibrio</jats:italic> -specific features, the T ring and H ring, but also provided the first structural evidence that the T ring interacts directly with the periplasmic domain of the stator. Docking atomic structures of key components into the <jats:italic>in situ</jats:italic> motor map allowed us to visualize the pseudoatomic architecture of the polar sheathed flagellum in <jats:italic>Vibrio</jats:italic> spp. and provides novel insight into its assembly and function. </jats:p>

<i>In Situ</i> Structure of the <i>Vibrio</i> Polar Flagellum Reveals a Distinct Outer Membrane Complex and Its Specific Interaction with the Stator

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