Transcriptional autoregulation of zebrafish <i>tbx6</i> is required for somite segmentation
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- Hiroyuki Ban
- Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
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- Daisuke Yokota
- Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
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- Shiori Otosaka
- Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
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- Morimichi Kikuchi
- Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
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- Hirofumi Kinoshita
- Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
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- Yuuri Fujino
- Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
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- Taijiro Yabe
- Exploratory Research Center on Life and Living Systems & National Institute for Basic Biology, National Institutes of Natural Sciences, Aichi 444-8787, Japan
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- Hiroki Ovara
- Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
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- Ayaka Izuka
- Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
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- Kagari Akama
- Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
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- Kyo Yamasu
- Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
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- Shinji Takada
- Exploratory Research Center on Life and Living Systems & National Institute for Basic Biology, National Institutes of Natural Sciences, Aichi 444-8787, Japan
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- Akinori Kawamura
- Division of Life Science, Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
抄録
<jats:p>The presumptive somite boundary in the presomitic mesoderm (PSM) is defined by the anterior border of the expression domain of Tbx6 protein. During somite segmentation, the expression domain of Tbx6 is regressed by Ripply-meditated degradation of Tbx6 protein. Although the expression of zebrafish tbx6 remains restricted to the PSM, the transcriptional regulation of tbx6 remains poorly understood. Here, we show that the expression of zebrafish tbx6 is maintained by transcriptional autoregulation. We find that a proximal-located cis-regulatory module, TR1, which contains two putative T-box sites, is required for somite segmentation in the intermediate body and for proper expression of segmentation genes. Embryos with deletion of TR1 exhibit significant reduction of tbx6 expression at the 12-somite stage, although its expression is initially observed. Additionally, Tbx6 is associated with TR1 and activates its own expression in the anterior PSM. Furthermore, the anterior expansion of tbx6 expression in ripply mutants is suppressed in a TR1-dependent manner. The results suggest that the autoregulatory loop of zebrafish tbx6 facilitates immediate removal of Tbx6 protein through termination of its own transcription at the anterior PSM.</jats:p>
収録刊行物
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- Development
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Development 146 dev177063-, 2019-01-01
The Company of Biologists
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詳細情報 詳細情報について
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- CRID
- 1360849946977115392
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- ISSN
- 14779129
- 09501991
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- データソース種別
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- Crossref
- KAKEN