Coexpressed subunits of dual genetic origin define a conserved supercomplex mediating essential protein import into chloroplasts

DOI DOI Web Site Web Site PubMed Research Data 3 Citations 85 References Open Access
  • Silvia Ramundo
    Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143;
  • Yukari Asakura
    Laboratory of Organelle Biology, Institute for Protein Research, Osaka University, Osaka 565-0871, Japan;
  • Patrice A. Salomé
    Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095;
  • Daniela Strenkert
    Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095;
  • Morgane Boone
    Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143;
  • Luke C. M. Mackinder
    Department of Biology, University of York, York YO10 5DD, United Kingdom;
  • Kazuaki Takafuji
    Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan;
  • Emine Dinc
    Department of Molecular Biology, University of Geneva, Geneva CH-1211, Switzerland;
  • Michèle Rahire
    Department of Molecular Biology, University of Geneva, Geneva CH-1211, Switzerland;
  • Michèle Crèvecoeur
    Department of Molecular Biology, University of Geneva, Geneva CH-1211, Switzerland;
  • Leonardo Magneschi
    Institute of Plant Biology and Biotechnology, University of Münster, Münster 48143, Germany;
  • Olivier Schaad
    Department of Biochemistry, University of Geneva, Geneva CH-1211, Switzerland;
  • Michael Hippler
    Institute of Plant Biology and Biotechnology, University of Münster, Münster 48143, Germany;
  • Martin C. Jonikas
    Howard Hughes Medical Institute, Chevy Chase, MD 20815;
  • Sabeeha Merchant
    Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095;
  • Masato Nakai
    Laboratory of Organelle Biology, Institute for Protein Research, Osaka University, Osaka 565-0871, Japan;
  • Jean-David Rochaix
    Department of Molecular Biology, University of Geneva, Geneva CH-1211, Switzerland;
  • Peter Walter
    Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143;

Description

<jats:title>Significance</jats:title> <jats:p> Chloroplasts are of vital importance in photosynthetic eukaryotic organisms. Like mitochondria, they contain their own genomes. Nevertheless, most chloroplast proteins are encoded by nuclear genes, translated in the cytosol, and must cross the chloroplast envelope membranes to reach their proper destinations inside the organelle. Despite its fundamental role, our knowledge of the machinery catalyzing chloroplast protein import is incomplete and controversial. Here, we address the evolutionary conservation, composition, and function of the chloroplast protein import machinery using the green alga <jats:italic>Chlamydomonas reinhardtii</jats:italic> as a model system. Our findings help clarify the current debate regarding the composition of the chloroplast protein import machinery, provide evidence for cross-compartmental coordination of its biogenesis, and open promising avenues for its structural characterization. </jats:p>

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