Synchrotron micro-X-ray fluorescence imaging of arsenic in frozen-hydrated sections of a root of <i>Pteris vittata</i>
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- Teruhiko Kashiwabara
- Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushimacho, Yokosuka, Kanagawa 237-0068, Japan
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- Nobuyuki Kitajima
- Fujita Co., 2025-1 Ono, Atsugi, Kanagawa 243-0125, Japan
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- Ryoko Onuma
- Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 162-8601, Japan
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- Naoki Fukuda
- Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 162-8601, Japan
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- Satoshi Endo
- Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 162-8601, Japan
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- Yasuko Terada
- SPring-8, Japan Synchrotron Radiation Research Institute (JASRI), Sayo-cho, Hyogo 679-5198, Japan
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- Tomoko Abe
- RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
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- Akiko Hokura
- Department of Applied Chemistry, School of Engineering, Tokyo Denki University, 5 Senju-Asahicho, Adachi, Tokyo 120-8551, Japan
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- Izumi Nakai
- Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 162-8601, Japan
説明
<jats:title>Abstract</jats:title> <jats:p>We performed micro-X-ray fluorescence imaging of frozen-hydrated sections of a root of Pteris vittata for the first time, to the best of our knowledge, to reveal the mechanism of arsenic (As) uptake. The As distribution was successfully visualized in cross sections of different parts of the root, which showed that (i) the major pathway of As uptake changes from symplastic to apoplastic transport in the direction of root growth, and (ii) As and K have different mobilities around the stele before xylem loading, despite their similar distributions outside the stele in the cross sections. These data can reasonably explain As reduction, axially observed around the root tip in the direction of root growth and radially observed in the endodermis in the cross sections, as a consequence of the incorporation of As into the cells or symplast of the root. In addition, previous observations of As species in the midrib can be reconciled by ascribing a reduction capacity to the root cells, which implies that As reduction mechanisms at the cellular level may be an important control on the peculiar root-to-shoot transport of As in P. vittata.</jats:p>
収録刊行物
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- Metallomics
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Metallomics 13 (4), mfab009-, 2021-03-09
Oxford University Press (OUP)
