Human retroviral antisense mRNAs are retained in the nuclei of infected cells for viral persistence
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- Guangyong Ma
- Institute of Pharmaceutical Science, China Pharmaceutical University, Nanjing 210009, China;
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- Jun-ichirou Yasunaga
- Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto 606-8507, Japan;
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- Kazuya Shimura
- Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto 606-8507, Japan;
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- Keiko Takemoto
- Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto 606-8507, Japan;
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- Miho Watanabe
- Department of Hematology, Rheumatology and Infectious Diseases, Kumamoto University School of Medicine, Kumamoto 860-8556, Japan
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- Masayuki Amano
- Department of Hematology, Rheumatology and Infectious Diseases, Kumamoto University School of Medicine, Kumamoto 860-8556, Japan
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- Hirotomo Nakata
- Department of Hematology, Rheumatology and Infectious Diseases, Kumamoto University School of Medicine, Kumamoto 860-8556, Japan
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- Benquan Liu
- Institute of Pharmaceutical Science, China Pharmaceutical University, Nanjing 210009, China;
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- Xiaorui Zuo
- Institute of Pharmaceutical Science, China Pharmaceutical University, Nanjing 210009, China;
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- Masao Matsuoka
- Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto 606-8507, Japan;
Description
<jats:title>Significance</jats:title> <jats:p> Two deadly human retroviruses, human T cell leukemia virus type 1 (HTLV-1) and HIV type 1 (HIV-1), enter latency in vivo, rendering viral countermeasures ineffective. Recently, novel retroviral genes have been discovered to be expressed from the antisense strand of retroviruses even during latency; they are called antisense genes, including the <jats:italic>HBZ</jats:italic> gene for HTLV-1 and <jats:italic>ASP</jats:italic> gene for HIV-1. We employed RNA-fluorescence in situ hybridization technology and discovered that human retroviral antisense messenger RNAs (mRNAs) are predominantly localized in the nucleus of infected cells, despite their coding function. Moreover, human retroviral antisense mRNAs are constantly expressed in latent retroviruses and retained in nucleus to support retroviral persistence; this may allow them to become novel feasible targets for retrovirus elimination. </jats:p>
Journal
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 118 (17), e2014783118-, 2021-04-19
Proceedings of the National Academy of Sciences
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Details 詳細情報について
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- CRID
- 1360853567636012928
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- ISSN
- 10916490
- 00278424
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- Data Source
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- Crossref
- KAKEN