Exocyst subunit Exo70B2 is linked to immune signaling and autophagy

DOI PDF PDF PubMed 被引用文献1件 参考文献73件
  • Carla Brillada
    Faculty of Biology, Cell Biology, University of Freiburg, 79104 Freiburg, Germany
  • Ooi-Kock Teh
    Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany
  • Franck Anicet Ditengou
    Faculty of Biology, Cell Biology, University of Freiburg, 79104 Freiburg, Germany
  • Chil-Woo Lee
    Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany
  • Till Klecker
    Institute of Cell Biology, University of Bayreuth, 95440 Bayreuth, Germany
  • Bushra Saeed
    Faculty of Biology, Cell Biology, University of Freiburg, 79104 Freiburg, Germany
  • Giulia Furlan
    Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany
  • Marco Zietz
    Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany
  • Gerd Hause
    Biozentrum, Martin-Luther-University Halle-Wittenberg, Halle 06120 (Saale), Germany
  • Lennart Eschen-Lippold
    Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany
  • Wolfgang Hoehenwarter
    Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany
  • Justin Lee
    Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany
  • Thomas Ott
    Faculty of Biology, Cell Biology, University of Freiburg, 79104 Freiburg, Germany
  • Marco Trujillo
    Faculty of Biology, Cell Biology, University of Freiburg, 79104 Freiburg, Germany

説明

<jats:title>Abstract</jats:title><jats:p>During the immune response, activation of the secretory pathway is key to mounting an effective response, while gauging its output is important to maintain cellular homeostasis. The Exo70 subunit of the exocyst functions as a spatiotemporal regulator by mediating numerous interactions with proteins and lipids. However, a molecular understanding of the exocyst regulation remains challenging. We show that, in Arabidopsis thaliana, Exo70B2 behaves as a bona fide exocyst subunit. Conversely, treatment with the salicylic acid (SA) defence hormone analog benzothiadiazole (BTH), or the immunogenic peptide flg22, induced Exo70B2 transport into the vacuole. We reveal that Exo70B2 interacts with AUTOPHAGY-RELATED PROTEIN 8 (ATG8) via two ATG8-interacting motives (AIMs) and its transport into the vacuole is dependent on autophagy. In line with its role in immunity, we discovered that Exo70B2 interacted with and was phosphorylated by the kinase MPK3. Mimicking phosphorylation had a dual impact on Exo70B2: first, by inhibiting localization at sites of active secretion, and second, it increased the interaction with ATG8. Phosphonull variants displayed higher effector-triggered immunity (ETI) and were hypersensitive to BTH, which induce secretion and autophagy. Our results suggest a molecular mechanism by which phosphorylation diverts Exo70B2 from the secretory into the autophagy pathway for its degradation, to dampen secretory activity.</jats:p>

収録刊行物

  • The Plant Cell

    The Plant Cell 33 (2), 404-419, 2020-12-03

    Oxford University Press (OUP)

被引用文献 (1)*注記

もっと見る

参考文献 (73)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

問題の指摘

ページトップへ