Differential mode of cholesterol inclusion with 2‐hydroxypropyl‐cyclodextrins increases safety margin in treatment of Niemann‐Pick disease type C
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- Yusei Yamada
- Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences Kumamoto University Kumamoto Japan
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- Yoichi Ishitsuka
- Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences Kumamoto University Kumamoto Japan
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- Yuki Kondo
- Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences Kumamoto University Kumamoto Japan
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- Shuichi Nakahara
- Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences Kumamoto University Kumamoto Japan
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- Asami Nishiyama
- Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences Kumamoto University Kumamoto Japan
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- Toru Takeo
- Division of Reproductive Engineering, Center for Animal Resources and Development (CARD) Kumamoto University Kumamoto Japan
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- Naomi Nakagata
- Division of Reproductive Engineering, Center for Animal Resources and Development (CARD) Kumamoto University Kumamoto Japan
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- Keiichi Motoyama
- Department of Physical Pharmaceutics, Graduate School of Pharmaceutical Sciences Kumamoto University Kumamoto Japan
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- Taishi Higashi
- Department of Physical Pharmaceutics, Graduate School of Pharmaceutical Sciences Kumamoto University Kumamoto Japan
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- Hidetoshi Arima
- Laboratory of Evidence‐Based Pharmacotherapy Daiichi University of Pharmacy Fukuoka Japan
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- Shunsuke Kamei
- Department of Molecular Medicine, Graduate School of Pharmaceutical Sciences Graduate School of Pharmaceutical Sciences Kumamoto Japan
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- Tsuyoshi Shuto
- Department of Molecular Medicine, Graduate School of Pharmaceutical Sciences Graduate School of Pharmaceutical Sciences Kumamoto Japan
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- Hirofumi Kai
- Department of Molecular Medicine, Graduate School of Pharmaceutical Sciences Graduate School of Pharmaceutical Sciences Kumamoto Japan
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- Yuji Hayashino
- Department of Bioinformatics, College of Life Sciences Ritsumeikan University Kusatsu Shiga Japan
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- Masatake Sugita
- Department of Bioinformatics, College of Life Sciences Ritsumeikan University Kusatsu Shiga Japan
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- Takeshi Kikuchi
- Department of Bioinformatics, College of Life Sciences Ritsumeikan University Kusatsu Shiga Japan
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- Fumio Hirata
- Toyota Physical and Chemical Research Institute Nagakute Aichi Japan
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- Toru Miwa
- Department of Otolaryngology‐Head and Neck Surgery Tazuke Kofukai Medical Research Institute, Kitano Hospital Osaka Japan
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- Hiroki Takeda
- Department of Otolaryngology‐Head and Neck Surgery Kumamoto University Kumamoto Japan
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- Yorihisa Orita
- Department of Otolaryngology‐Head and Neck Surgery Kumamoto University Kumamoto Japan
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- Takahiro Seki
- Department of Chemico‐Pharmacological Sciences, Graduate School of Pharmaceutical Sciences Kumamoto University Kumamoto Japan
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- Tomoko Ohta
- Department of Chemico‐Pharmacological Sciences, Graduate School of Pharmaceutical Sciences Kumamoto University Kumamoto Japan
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- Yuki Kurauchi
- Department of Chemico‐Pharmacological Sciences, Graduate School of Pharmaceutical Sciences Kumamoto University Kumamoto Japan
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- Hiroshi Katsuki
- Department of Chemico‐Pharmacological Sciences, Graduate School of Pharmaceutical Sciences Kumamoto University Kumamoto Japan
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- Muneaki Matsuo
- Department of Pediatrics, Faculty of Medicine Saga University Saga Japan
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- Katsumi Higaki
- Research Initiative Center, Organization for Research Initiative and Promotion Tottori University Yonago Japan
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- Kousaku Ohno
- Sanin Rosai Hospital Yonago Japan
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- Shirou Matsumoto
- Department of Pediatrics, Graduate School of Medical Sciences Kumamoto University Kumamoto Japan
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- Takumi Era
- Department of Cell Modulation, Institute of Molecular Embryology and Genetics Kumamoto University Kumamoto Japan
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- Tetsumi Irie
- Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences Kumamoto University Kumamoto Japan
抄録
<jats:sec><jats:title>Background and Purpose</jats:title><jats:p>Niemann‐Pick disease type C (NPC) is a lysosomal storage disorder with disrupted intracellular cholesterol trafficking. A cyclic heptasaccharide, 2‐hydroxypropyl‐β‐cyclodextrin (HP‐β‐CD), is a cholesterol solubilizer that is being developed to treat NPC, but its ototoxicity and pulmonary toxicity remain important issues. We have characterized 2‐hydroxypropyl‐γ‐cyclodextrin (HP‐γ‐CD), a cyclic octasaccharide with a larger cavity than HP‐β‐CD, as a candidate drug to treat NPC. However, the molecular target of HP‐γ‐CD with respect to NPC and its potential for clinical application are still unclear.</jats:p></jats:sec><jats:sec><jats:title>Experimental Approach</jats:title><jats:p>We investigated the mode of interaction between HP‐γ‐CD and cholesterol by phase‐solubility analysis, proton NMR spectroscopy and molecular dynamics simulations. We then evaluated the therapeutic effects of HP‐γ‐CD compared with HP‐β‐CD using cellular and murine NPC models. Mouse auditory and pulmonary function tests were also conducted.</jats:p></jats:sec><jats:sec><jats:title>Key Results</jats:title><jats:p>HP‐γ‐CD solely formed a 1:1 inclusion complex with cholesterol with an affinity similar to that of HP‐β‐CD. In vitro, HP‐γ‐CD and HP‐β‐CD amelioration of NPC‐related manifestations was almost equivalent at lower concentrations. However, at higher concentrations, the cholesterol inclusion mode of HP‐β‐CD shifted to the highly soluble 2:1 complex whereas that of HP‐γ‐CD maintained solely the 1:1 complex. The constant lower cholesterol solubilizing ability of HP‐γ‐CD conferred it with significantly reduced toxicity compared with HP‐β‐CD, but equal efficacy in treating a mouse model of NPC.</jats:p></jats:sec><jats:sec><jats:title>Conclusions and Implications</jats:title><jats:p>HP‐γ‐CD can serve as a fine‐tuned cholesterol solubilizer for the treatment of NPC with a wider safety margin than HP‐β‐CD in terms of ototoxicity and pulmonary toxicity.</jats:p></jats:sec>
収録刊行物
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- British Journal of Pharmacology
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British Journal of Pharmacology 178 (13), 2727-2746, 2021-05-12
Wiley
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キーワード
詳細情報 詳細情報について
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- CRID
- 1360853567817341568
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- ISSN
- 14765381
- 00071188
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- データソース種別
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- Crossref
- KAKEN