Rapid Amplification of Plasmid and Phage DNA Using Phi29 DNA Polymerase and Multiply-Primed Rolling Circle Amplification

DOI Web Site 被引用文献31件

説明

<jats:p>We describe a simple method of using rolling circle amplification to amplify vector DNA such as M13 or plasmid DNA from single colonies or plaques. Using random primers and φ29 DNA polymerase, circular DNA templates can be amplified 10,000-fold in a few hours. This procedure removes the need for lengthy growth periods and traditional DNA isolation methods. Reaction products can be used directly for DNA sequencing after phosphatase treatment to inactivate unincorporated nucleotides. Amplified products can also be used for in vitro cloning, library construction, and other molecular biology applications.</jats:p>

収録刊行物

  • Genome Research

    Genome Research 11 (6), 1095-1099, 2001-06-01

    Cold Spring Harbor Laboratory

被引用文献 (31)*注記

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ