The long pentraxin PTX3 binds to apoptotic cells and regulates their clearance by antigen-presenting dendritic cells

DOI Web Site 2 Citations
  • Patrizia Rovere
    From the Tumor Immunology Laboratory and Cancer Immunotherapy and Gene Therapy Program, Istituto Scientifico H S. Raffaele and Vita-Salute San Raffaele University; Department of Biology, University of Milan and Istituto di Ricerche Farmacologiche “Mario Negri,” Milan, Italy; and the Sezione di Patologia e Immunologia, Dipartimento di Biotecnologie, Università di Brescia, Brescia, Italy.
  • Giuseppe Peri
    From the Tumor Immunology Laboratory and Cancer Immunotherapy and Gene Therapy Program, Istituto Scientifico H S. Raffaele and Vita-Salute San Raffaele University; Department of Biology, University of Milan and Istituto di Ricerche Farmacologiche “Mario Negri,” Milan, Italy; and the Sezione di Patologia e Immunologia, Dipartimento di Biotecnologie, Università di Brescia, Brescia, Italy.
  • Fausto Fazzini
    From the Tumor Immunology Laboratory and Cancer Immunotherapy and Gene Therapy Program, Istituto Scientifico H S. Raffaele and Vita-Salute San Raffaele University; Department of Biology, University of Milan and Istituto di Ricerche Farmacologiche “Mario Negri,” Milan, Italy; and the Sezione di Patologia e Immunologia, Dipartimento di Biotecnologie, Università di Brescia, Brescia, Italy.
  • Barbara Bottazzi
    From the Tumor Immunology Laboratory and Cancer Immunotherapy and Gene Therapy Program, Istituto Scientifico H S. Raffaele and Vita-Salute San Raffaele University; Department of Biology, University of Milan and Istituto di Ricerche Farmacologiche “Mario Negri,” Milan, Italy; and the Sezione di Patologia e Immunologia, Dipartimento di Biotecnologie, Università di Brescia, Brescia, Italy.
  • Andrea Doni
    From the Tumor Immunology Laboratory and Cancer Immunotherapy and Gene Therapy Program, Istituto Scientifico H S. Raffaele and Vita-Salute San Raffaele University; Department of Biology, University of Milan and Istituto di Ricerche Farmacologiche “Mario Negri,” Milan, Italy; and the Sezione di Patologia e Immunologia, Dipartimento di Biotecnologie, Università di Brescia, Brescia, Italy.
  • Attilio Bondanza
    From the Tumor Immunology Laboratory and Cancer Immunotherapy and Gene Therapy Program, Istituto Scientifico H S. Raffaele and Vita-Salute San Raffaele University; Department of Biology, University of Milan and Istituto di Ricerche Farmacologiche “Mario Negri,” Milan, Italy; and the Sezione di Patologia e Immunologia, Dipartimento di Biotecnologie, Università di Brescia, Brescia, Italy.
  • Valérie S. Zimmermann
    From the Tumor Immunology Laboratory and Cancer Immunotherapy and Gene Therapy Program, Istituto Scientifico H S. Raffaele and Vita-Salute San Raffaele University; Department of Biology, University of Milan and Istituto di Ricerche Farmacologiche “Mario Negri,” Milan, Italy; and the Sezione di Patologia e Immunologia, Dipartimento di Biotecnologie, Università di Brescia, Brescia, Italy.
  • Cecilia Garlanda
    From the Tumor Immunology Laboratory and Cancer Immunotherapy and Gene Therapy Program, Istituto Scientifico H S. Raffaele and Vita-Salute San Raffaele University; Department of Biology, University of Milan and Istituto di Ricerche Farmacologiche “Mario Negri,” Milan, Italy; and the Sezione di Patologia e Immunologia, Dipartimento di Biotecnologie, Università di Brescia, Brescia, Italy.
  • Umberto Fascio
    From the Tumor Immunology Laboratory and Cancer Immunotherapy and Gene Therapy Program, Istituto Scientifico H S. Raffaele and Vita-Salute San Raffaele University; Department of Biology, University of Milan and Istituto di Ricerche Farmacologiche “Mario Negri,” Milan, Italy; and the Sezione di Patologia e Immunologia, Dipartimento di Biotecnologie, Università di Brescia, Brescia, Italy.
  • Maria Grazia Sabbadini
    From the Tumor Immunology Laboratory and Cancer Immunotherapy and Gene Therapy Program, Istituto Scientifico H S. Raffaele and Vita-Salute San Raffaele University; Department of Biology, University of Milan and Istituto di Ricerche Farmacologiche “Mario Negri,” Milan, Italy; and the Sezione di Patologia e Immunologia, Dipartimento di Biotecnologie, Università di Brescia, Brescia, Italy.
  • Claudio Rugarli
    From the Tumor Immunology Laboratory and Cancer Immunotherapy and Gene Therapy Program, Istituto Scientifico H S. Raffaele and Vita-Salute San Raffaele University; Department of Biology, University of Milan and Istituto di Ricerche Farmacologiche “Mario Negri,” Milan, Italy; and the Sezione di Patologia e Immunologia, Dipartimento di Biotecnologie, Università di Brescia, Brescia, Italy.
  • Alberto Mantovani
    From the Tumor Immunology Laboratory and Cancer Immunotherapy and Gene Therapy Program, Istituto Scientifico H S. Raffaele and Vita-Salute San Raffaele University; Department of Biology, University of Milan and Istituto di Ricerche Farmacologiche “Mario Negri,” Milan, Italy; and the Sezione di Patologia e Immunologia, Dipartimento di Biotecnologie, Università di Brescia, Brescia, Italy.
  • Angelo A. Manfredi
    From the Tumor Immunology Laboratory and Cancer Immunotherapy and Gene Therapy Program, Istituto Scientifico H S. Raffaele and Vita-Salute San Raffaele University; Department of Biology, University of Milan and Istituto di Ricerche Farmacologiche “Mario Negri,” Milan, Italy; and the Sezione di Patologia e Immunologia, Dipartimento di Biotecnologie, Università di Brescia, Brescia, Italy.

Bibliographic Information

Published
2000-12-15
DOI
  • 10.1182/blood.v96.13.4300.h8004300_4300_4306
Publisher
American Society of Hematology

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Description

<jats:p>Pentraxins are acute-phase proteins produced in vivo during inflammatory reactions. Classical short pentraxins, C-reactive protein, and serum amyloid P component are generated in the liver in response to interleukin (IL)–6. The long pentraxin PTX3 is produced in tissues under the control of primary proinflammatory signals, such as lipopolysaccharide, IL-1β, and tumor necrosis factor-α, which also promote maturation of dendritic cells (DCs). Cell death commonly occurs during inflammatory reactions. In this study, it is shown that PTX3 specifically binds to dying cells. The binding was dose dependent and saturable. Recognition was restricted to extranuclear membrane domains and to a chronological window after UV irradiation or after CD95 cross-linking–induced or spontaneous cell death in vitro. PTX3 bound to necrotic cells to a lesser extent. Human DCs failed to internalize dying cells in the presence of PTX3, while they took up normally soluble or inert particulate substrates. These results suggest that PTX3 sequesters cell remnants from antigen-presenting cells, possibly contributing to preventing the onset of autoimmune reactions in inflamed tissues.</jats:p>

Journal

  • Blood

    Blood 96 (13), 4300-4306, 2000-12-15

    American Society of Hematology

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