Distribution and accumulation of dietary ergothioneine and its metabolites in mouse tissues
説明
<jats:title>Abstract</jats:title><jats:p>L-ergothioneine (ET) is a diet-derived amino acid that accumulates at high concentrations in animals and humans. Numerous studies have highlighted its antioxidant abilities <jats:italic>in vitro</jats:italic>, and possible cytoprotective capabilities <jats:italic>in vivo</jats:italic>. We investigated the uptake and distribution of ET in various organs by a highly sensitive and specific liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) technique, both before and after oral administration of pure ET (35 and 70 mg/kg/day for 1, 7, and 28 days) to male C57BL6J mice. ET primarily concentrates in the liver and whole blood, and also in spleen, kidney, lung, heart, intestines, eye, and brain tissues. Strong correlations were found between ET and its putative metabolites - hercynine, ET-sulfonate (ET-SO<jats:sub>3</jats:sub>H), and S-methyl ET. Hercynine accumulates in the brain after prolonged ET administration. This study demonstrates the uptake and distribution of ET and provides a foundation for future studies with ET to target oxidative damage in a range of tissues in human diseases.</jats:p>
収録刊行物
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- Scientific Reports
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Scientific Reports 8 (1), 1-, 2018-01-25
Springer Science and Business Media LLC
