Molecular, spatial, and functional single-cell profiling of the hypothalamic preoptic region

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  • Jeffrey R. Moffitt
    Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.
  • Dhananjay Bambah-Mukku
    Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.
  • Stephen W. Eichhorn
    Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.
  • Eric Vaughn
    Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.
  • Karthik Shekhar
    Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA 02139, USA.
  • Julio D. Perez
    Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.
  • Nimrod D. Rubinstein
    Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.
  • Junjie Hao
    Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.
  • Aviv Regev
    Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.
  • Catherine Dulac
    Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.
  • Xiaowei Zhuang
    Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA.

Abstract

<jats:title>Mapping the brain, one neuron at a time</jats:title> <jats:p> Spatial transcriptomics can link molecularly described cell types to their anatomical positions and functional roles. Moffitt <jats:italic>et al.</jats:italic> used a combination of single-cell RNA-sequencing and MERFISH (multiplexed error-robust fluorescence in situ hybridization) to map the identity and location of specific cell types within the mouse preoptic hypothalamus and surrounding areas of the brain (see the Perspective by Tasic and Nicovich). They related these cell types to specific behaviors via gene activity. The approach provides an unbiased description of cell types of the preoptic area, which are important for sleep, thermoregulation, thirst, and social behavior. </jats:p> <jats:p> <jats:italic>Science</jats:italic> , this issue p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" related-article-type="in-this-issue" xlink:href="10.1126/science.aau5324">eaau5324</jats:related-article> ; see also p. <jats:related-article xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="doi" issue="6416" page="749" related-article-type="in-this-issue" vol="362" xlink:href="10.1126/science.aav4841">749</jats:related-article> </jats:p>

Journal

  • Science

    Science 362 (6416), eaau5324-, 2018-11-16

    American Association for the Advancement of Science (AAAS)

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