<i>In Vivo</i> Dynamic MRI Tracking of Rat T‐Cells Labeled with Superparamagnetic Iron‐Oxide Particles
説明
<jats:title>Abstract</jats:title><jats:p>Dynamic MRI tracking of rat T‐cells <jats:italic>in vivo</jats:italic> is performed in rat testicles after labeling isolated rat T‐cells <jats:italic>in vitro</jats:italic> with superparamagnetic dextran‐coated iron‐oxide particles, BMS180549. Tissue inflammation induced by the local injection of the calcium ionophore, A23187, is used to attract labeled T‐cells. Gradient‐echo MR images of rat testicles show a statistically significant decrease (4%) of the signal intensity in areas of injection of A23187 as early as 30 min after intravenous infusion of 2 x 10<jats:sup>8</jats:sup> labeled T‐cells. The signal change reaches its maximum (6–7% decrease) at about 60–120 min after cell infusion. <jats:italic>T</jats:italic><jats:sub>2</jats:sub>‐mapping also shows a decrease of <jats:italic>T</jats:italic><jats:sub>2</jats:sub> in the areas with A23187. Image quantitation, which includes a chemical‐shift effect, significantly enhances the sensitivity for detection of superparamagnetically labeled T‐cells. Localization of labeled T‐cells in rat testicles has been verified by fluorescence microscopy studies of T‐cells co‐labeled with a lipophilic fluorescent carbocyanine dye, 1,1‐dioctadecyl‐3,3,3',3'‐tetramethyl‐lindocarbocyanine perchlorate. These results represent the first successful demonstration of dynamic tracking of specific cells <jats:italic>in vivo</jats:italic> using MRI.</jats:p>
収録刊行物
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- Magnetic Resonance in Medicine
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Magnetic Resonance in Medicine 33 (2), 200-208, 1995-02
Wiley