Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System
説明
<jats:title>Abstract</jats:title><jats:p>The myostatin (MSTN) gene is important because of its role in regulation of skeletal muscle growth in all vertebrates. In this study, CRISPR/Cas9 was utilized to successfully target the channel catfish, <jats:italic>Ictalurus punctatus</jats:italic>, muscle suppressor gene MSTN. CRISPR/Cas9 induced high rates (88–100%) of mutagenesis in the target protein-encoding sites of MSTN. MSTN-edited fry had more muscle cells (<jats:italic>p</jats:italic> < 0.001) than controls, and the mean body weight of gene-edited fry increased by 29.7%. The nucleic acid alignment of the mutated sequences against the wild-type sequence revealed multiple insertions and deletions. These results demonstrate that CRISPR/Cas9 is a highly efficient tool for editing the channel catfish genome, and opens ways for facilitating channel catfish genetic enhancement and functional genomics. This approach may produce growth-enhanced channel catfish and increase productivity.</jats:p>
収録刊行物
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- Scientific Reports
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Scientific Reports 7 (1), 7301-, 2017-08-04
Springer Science and Business Media LLC
