Network Inference Analysis Identifies an <i>APRR2-Like</i> Gene Linked to Pigment Accumulation in Tomato and Pepper Fruits
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- Yu Pan
- Division of Plant and Crop Sciences, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, United Kingdom (Y.P., G.Br., K.P., C.L., R.F., A.M., S.J., N.H.C., C.H., G.B.S.); School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS, United Kingdom (G.Ba.); Syngenta Seeds, Jealott’s Hill International Research Station, Bracknell, Berkshire RG42 6EY, United King
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- Glyn Bradley
- Division of Plant and Crop Sciences, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, United Kingdom (Y.P., G.Br., K.P., C.L., R.F., A.M., S.J., N.H.C., C.H., G.B.S.); School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS, United Kingdom (G.Ba.); Syngenta Seeds, Jealott’s Hill International Research Station, Bracknell, Berkshire RG42 6EY, United King
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- Kevin Pyke
- Division of Plant and Crop Sciences, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, United Kingdom (Y.P., G.Br., K.P., C.L., R.F., A.M., S.J., N.H.C., C.H., G.B.S.); School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS, United Kingdom (G.Ba.); Syngenta Seeds, Jealott’s Hill International Research Station, Bracknell, Berkshire RG42 6EY, United King
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- Graham Ball
- Division of Plant and Crop Sciences, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, United Kingdom (Y.P., G.Br., K.P., C.L., R.F., A.M., S.J., N.H.C., C.H., G.B.S.); School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS, United Kingdom (G.Ba.); Syngenta Seeds, Jealott’s Hill International Research Station, Bracknell, Berkshire RG42 6EY, United King
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- Chungui Lu
- Division of Plant and Crop Sciences, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, United Kingdom (Y.P., G.Br., K.P., C.L., R.F., A.M., S.J., N.H.C., C.H., G.B.S.); School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS, United Kingdom (G.Ba.); Syngenta Seeds, Jealott’s Hill International Research Station, Bracknell, Berkshire RG42 6EY, United King
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- Rupert Fray
- Division of Plant and Crop Sciences, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, United Kingdom (Y.P., G.Br., K.P., C.L., R.F., A.M., S.J., N.H.C., C.H., G.B.S.); School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS, United Kingdom (G.Ba.); Syngenta Seeds, Jealott’s Hill International Research Station, Bracknell, Berkshire RG42 6EY, United King
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- Alexandra Marshall
- Division of Plant and Crop Sciences, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, United Kingdom (Y.P., G.Br., K.P., C.L., R.F., A.M., S.J., N.H.C., C.H., G.B.S.); School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS, United Kingdom (G.Ba.); Syngenta Seeds, Jealott’s Hill International Research Station, Bracknell, Berkshire RG42 6EY, United King
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- Subhalai Jayasuta
- Division of Plant and Crop Sciences, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, United Kingdom (Y.P., G.Br., K.P., C.L., R.F., A.M., S.J., N.H.C., C.H., G.B.S.); School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS, United Kingdom (G.Ba.); Syngenta Seeds, Jealott’s Hill International Research Station, Bracknell, Berkshire RG42 6EY, United King
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- Charles Baxter
- Division of Plant and Crop Sciences, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, United Kingdom (Y.P., G.Br., K.P., C.L., R.F., A.M., S.J., N.H.C., C.H., G.B.S.); School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS, United Kingdom (G.Ba.); Syngenta Seeds, Jealott’s Hill International Research Station, Bracknell, Berkshire RG42 6EY, United King
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- Rik van Wijk
- Division of Plant and Crop Sciences, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, United Kingdom (Y.P., G.Br., K.P., C.L., R.F., A.M., S.J., N.H.C., C.H., G.B.S.); School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS, United Kingdom (G.Ba.); Syngenta Seeds, Jealott’s Hill International Research Station, Bracknell, Berkshire RG42 6EY, United King
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- Laurie Boyden
- Division of Plant and Crop Sciences, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, United Kingdom (Y.P., G.Br., K.P., C.L., R.F., A.M., S.J., N.H.C., C.H., G.B.S.); School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS, United Kingdom (G.Ba.); Syngenta Seeds, Jealott’s Hill International Research Station, Bracknell, Berkshire RG42 6EY, United King
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- Rebecca Cade
- Division of Plant and Crop Sciences, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, United Kingdom (Y.P., G.Br., K.P., C.L., R.F., A.M., S.J., N.H.C., C.H., G.B.S.); School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS, United Kingdom (G.Ba.); Syngenta Seeds, Jealott’s Hill International Research Station, Bracknell, Berkshire RG42 6EY, United King
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- Natalie H. Chapman
- Division of Plant and Crop Sciences, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, United Kingdom (Y.P., G.Br., K.P., C.L., R.F., A.M., S.J., N.H.C., C.H., G.B.S.); School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS, United Kingdom (G.Ba.); Syngenta Seeds, Jealott’s Hill International Research Station, Bracknell, Berkshire RG42 6EY, United King
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- Paul D. Fraser
- Division of Plant and Crop Sciences, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, United Kingdom (Y.P., G.Br., K.P., C.L., R.F., A.M., S.J., N.H.C., C.H., G.B.S.); School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS, United Kingdom (G.Ba.); Syngenta Seeds, Jealott’s Hill International Research Station, Bracknell, Berkshire RG42 6EY, United King
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- Charlie Hodgman
- Division of Plant and Crop Sciences, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, United Kingdom (Y.P., G.Br., K.P., C.L., R.F., A.M., S.J., N.H.C., C.H., G.B.S.); School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS, United Kingdom (G.Ba.); Syngenta Seeds, Jealott’s Hill International Research Station, Bracknell, Berkshire RG42 6EY, United King
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- Graham B. Seymour
- Division of Plant and Crop Sciences, University of Nottingham, Sutton Bonington, Loughborough LE12 5RD, United Kingdom (Y.P., G.Br., K.P., C.L., R.F., A.M., S.J., N.H.C., C.H., G.B.S.); School of Science and Technology, Nottingham Trent University, Nottingham NG11 8NS, United Kingdom (G.Ba.); Syngenta Seeds, Jealott’s Hill International Research Station, Bracknell, Berkshire RG42 6EY, United King
抄録
<jats:title>Abstract</jats:title> <jats:p>Carotenoids represent some of the most important secondary metabolites in the human diet, and tomato (Solanum lycopersicum) is a rich source of these health-promoting compounds. In this work, a novel and fruit-related regulator of pigment accumulation in tomato has been identified by artificial neural network inference analysis and its function validated in transgenic plants. A tomato fruit gene regulatory network was generated using artificial neural network inference analysis and transcription factor gene expression profiles derived from fruits sampled at various points during development and ripening. One of the transcription factor gene expression profiles with a sequence related to an Arabidopsis (Arabidopsis thaliana) ARABIDOPSIS PSEUDO RESPONSE REGULATOR2-LIKE gene (APRR2-Like) was up-regulated at the breaker stage in wild-type tomato fruits and, when overexpressed in transgenic lines, increased plastid number, area, and pigment content, enhancing the levels of chlorophyll in immature unripe fruits and carotenoids in red ripe fruits. Analysis of the transcriptome of transgenic lines overexpressing the tomato APPR2-Like gene revealed up-regulation of several ripening-related genes in the overexpression lines, providing a link between the expression of this tomato gene and the ripening process. A putative ortholog of the tomato APPR2-Like gene in sweet pepper (Capsicum annuum) was associated with pigment accumulation in fruit tissues. We conclude that the function of this gene is conserved across taxa and that it encodes a protein that has an important role in ripening.</jats:p>
収録刊行物
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- Plant Physiology
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Plant Physiology 161 (3), 1476-1485, 2013-01-04
Oxford University Press (OUP)