Chemiluminescence method for the determination of adenine after reaction with phenylglyoxal

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Abstract A chemiluminescence (CL) method is described for the determination of adenine. Treatement of adenine with phenylglyoxal in acidic propan-2-ol gave products that produced CL in the presence of hydrogen peroxide, 2-mercaptoethanol and sodium hydroxide. The proposed method is highly sensitive and selective to adenine and adenosine. Adenine can be determined in the range 1.5 × 10 −4 −5.0 × 10 −7 M. The detection limit for adenine is 1.9 × 10 −7 M (19 pmol per assay).

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