Evidence for the organization of chromatin in megabase pair-sized loops arranged along a random walk path in the human G0/G1 interphase nucleus.
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- H Yokota
- Department of Molecular Biotechnology, University of Washington, Seattle 98195, USA.
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- G van den Engh
- Department of Molecular Biotechnology, University of Washington, Seattle 98195, USA.
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- J E Hearst
- Department of Molecular Biotechnology, University of Washington, Seattle 98195, USA.
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- R K Sachs
- Department of Molecular Biotechnology, University of Washington, Seattle 98195, USA.
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- B J Trask
- Department of Molecular Biotechnology, University of Washington, Seattle 98195, USA.
説明
<jats:p>We determined the folding of chromosomes in interphase nuclei by measuring the distance between points on the same chromosome. Over 25,000 measurements were made in G0/G1 nuclei between DNA sequences separated by 0.15-190 megabase pairs (Mbp) on three human chromosomes. The DNA sequences were specifically labeled by fluorescence in situ hybridization. The relationship between mean-square interphase distance and genomic separation has two linear phases, with a transition at approximately 2 Mbp. This biphasic relationship indicates the existence of two organizational levels at scales > 100 kbp. On one level, chromatin appears to be arranged in large loops several Mbp in size. Within each loop, chromatin is randomly folded. On the second level, specific loop-attachment sites are arranged to form a supple, backbonelike structure, which also shows characteristic random walk behavior. This random walk/giant loop model is the simplest model that fully describes the observed large-scale spatial relationships. Additional evidence for large loops comes from measurements among probes in Xq28, where interphase distance increases and then locally decreases with increasing genomic separation.</jats:p>
収録刊行物
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- The Journal of cell biology
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The Journal of cell biology 130 (6), 1239-1249, 1995-09-15
Rockefeller University Press
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詳細情報 詳細情報について
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- CRID
- 1360855570959793536
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- NII論文ID
- 30017410407
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- ISSN
- 15408140
- 00219525
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- データソース種別
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