The turnover of ribosomal RNA of Escherichia coli in a magnesium-deficient stage
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説明
Abstract 1. 1. Escherichia coli was grown in a magnesium-deficient medium. The ribosomal RNA was labeled, and followed during separation by chromatography on methylated bovine serum albumin. Marked degradation of ribosomal RNA was observed, although the total amount remained constant. This means that there was a rapid turnover of ribosomal RNA during growth. 2. The degradation products of ribosomal RNA during magnesium deficiency first appeared in the region of soluble RNA on methylated bovine serum albumin chromatograms, but were later released into the culture medium. 3. When pulse labeled with 32 P in the magnesium-deficient stage, no DNA-type RNA was obtained, but heterogeneous RNA's appeared with nucleotide compositions of the ribosomal RNA type. These RNA's were presumed to be precursors of ribosomal RNA.
収録刊行物
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- Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis
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Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis 114 (2), 245-253, 1966-02
Elsevier BV