Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases
説明
<jats:sec> <jats:title>Abstract</jats:title> <jats:p>Urea (Ua) is produced in DNA as the result of oxidative damage to thymine and guanine. It was previously reported that Klenow fragment (Kf) exo<jats:sup>−</jats:sup> incorporated dATP opposite Ua, and that DNA polymerase β was blocked by Ua. We report here the following nucleotide incorporations opposite Ua by various DNA polymerases: DNA polymerase α, dATP and dGTP (dATP > dGTP); DNA polymerase δ, dATP; DNA polymerase ζ, dATP; Kf exo<jats:sup>−</jats:sup>, dATP; <jats:italic>Sulfolobus solfataricus</jats:italic> P2 DNA polymerase IV (Dpo4), dGTP and dATP (dGTP > dATP); and DNA polymerase η, dCTP, dGTP, dATP, and dTTP (dCTP > dGTP > dATP > dTTP). DNA polymerases β and ε were blocked by Ua. Elongation by DNA polymerases δ and ζ stopped after inserting dATP opposite Ua. Importantly, the elongation efficiency to full-length beyond Ua using DNA polymerase η and Dpo4 were almost the same as that of natural DNA.</jats:p> </jats:sec><jats:sec> <jats:title>Graphical abstract</jats:title> </jats:sec>
収録刊行物
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- Genes and Environment
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Genes and Environment 44 (1), 2022-02-15
Springer Science and Business Media LLC