Regulation of Msh4-Msh5 association with meiotic chromosomes in budding yeast
-
- Krishnaprasad G Nandanan
- School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram, Trivandrum 695016, India
-
- Sagar Salim
- School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram, Trivandrum 695016, India
-
- Ajith V Pankajam
- School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram, Trivandrum 695016, India
-
- Miki Shinohara
- Graduate School of Agriculture, Kindai University, Nara 631-8505, Japan
-
- Gen Lin
- Genome Biology Unit, European Molecular Biology Laboratory, Heidelberg 69117, Germany
-
- Parijat Chakraborty
- School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram, Trivandrum 695016, India
-
- Amamah Farnaz
- School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram, Trivandrum 695016, India
-
- Lars M Steinmetz
- Genome Biology Unit, European Molecular Biology Laboratory, Heidelberg 69117, Germany
-
- Akira Shinohara
- Institute for Protein Research, Osaka University, Osaka 565-0871, Japan
-
- Koodali T Nishant
- School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram, Trivandrum 695016, India
Abstract
<jats:title>Abstract</jats:title> <jats:p>In the baker’s yeast Saccharomyces cerevisiae, most of the meiotic crossovers are generated through a pathway involving the highly conserved mismatch repair related Msh4-Msh5 complex. To understand the role of Msh4-Msh5 in meiotic crossing over, we determined its genome wide in vivo binding sites in meiotic cells. We show that Msh5 specifically associates with DSB hotspots, chromosome axes, and centromeres on chromosomes. A basal level of Msh5 association with these chromosomal features is observed even in the absence of DSB formation (spo11Δ mutant) at the early stages of meiosis. But efficient binding to DSB hotspots and chromosome axes requires DSB formation and resection and is enhanced by double Holliday junction structures. Msh5 binding is also correlated to DSB frequency and enhanced on small chromosomes with higher DSB and crossover density. The axis protein Red1 is required for Msh5 association with the chromosome axes and DSB hotspots but not centromeres. Although binding sites of Msh5 and other pro-crossover factors like Zip3 show extensive overlap, Msh5 associates with centromeres independent of Zip3. These results on Msh5 localization in wild type and meiotic mutants have implications for how Msh4-Msh5 works with other pro-crossover factors to ensure crossover formation.</jats:p>
Journal
-
- Genetics
-
Genetics 219 (2), 2021-07-08
Oxford University Press (OUP)
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1360857593706008960
-
- ISSN
- 19432631
-
- Data Source
-
- Crossref
- KAKEN