Functional assessment of miR‑1291 in colon cancer cells

  • Jiaqi Wang
    Department of Molecular Pathology, Division of Health Sciences, Osaka University, Suita, Osaka 565‑0871, Japan
  • Yuhki Yokoyama
    Department of Molecular Pathology, Division of Health Sciences, Osaka University, Suita, Osaka 565‑0871, Japan
  • Haruka Hirose
    Department of Molecular Pathology, Division of Health Sciences, Osaka University, Suita, Osaka 565‑0871, Japan
  • Yuki Shimomura
    Department of Molecular Pathology, Division of Health Sciences, Osaka University, Suita, Osaka 565‑0871, Japan
  • Saki Bonkobara
    Department of Molecular Pathology, Division of Health Sciences, Osaka University, Suita, Osaka 565‑0871, Japan
  • Hiroaki Itakura
    Department of Surgery and Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka 565‑0871, Japan
  • Shihori Kouda
    Department of Molecular Pathology, Division of Health Sciences, Osaka University, Suita, Osaka 565‑0871, Japan
  • Yoshihiro Morimoto
    Department of Surgery and Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka 565‑0871, Japan
  • Kazumasa Minami
    Department of Radiation Oncology, Graduate School of Medicine, Osaka University, Suita, Osaka 565‑0871, Japan
  • Hidekazu Takahashi
    Department of Surgery and Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka 565‑0871, Japan
  • Satoshi Shibata
    Department of Molecular Pathology, Division of Health Sciences, Osaka University, Suita, Osaka 565‑0871, Japan
  • Shogo Kobayashi
    Department of Surgery and Gastroenterological Surgery, Graduate School of Medicine, Osaka University,Suita, Osaka 565‑0871, Japan
  • Mamoru Uemura
    Department of Surgery and Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka 565‑0871, Japan
  • Susumu Tanaka
    First Department of Oral and Maxillofacial Surgery, Graduate School of Dentistry, Osaka University, Suita, Osaka 565‑0871, Japan
  • Xin Wu
    Department of Molecular Pathology, Division of Health Sciences, Osaka University, Suita, Osaka 565‑0871, Japan
  • Shinji Tanaka
    Department of Molecular Oncology, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo 113‑8510, Japan
  • Masaki Mori
    Tokai University, Graduate School of Medicine, Isehara, Kanagawa 259‑1193, Japan
  • Hirofumi Yamamoto
    Department of Molecular Pathology, Division of Health Sciences, Osaka University, Suita, Osaka 565‑0871, Japan

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説明

miR‑1291 exerts an anti‑tumor effect in a subset of human carcinomas, including pancreatic cancer. However, its role in colorectal cancer (CRC) is largely unknown. In the present study, the expression and effect of miR‑1291 in CRC cells was investigated. It was identified that miR‑1291 significantly suppressed the proliferation, invasion, cell mobility and colony formation of CRC cells. Additionally, miR‑1291 induced cell apoptosis. A luciferase reporter assay revealed that miR‑1291 directly bound the 3'‑untranslated region sequence of doublecortin‑like kinase 1 (DCLK1). miR‑1291 also suppressed DCLK1 mRNA and protein expression in HCT116 cells that expressed DCLK1. Furthermore, miR‑1291 suppressed cancer stem cell markers BMI1 and CD133, and inhibited sphere formation. The inhibitory effects on sphere formation, invasion and mobility in HCT116 cells were also explored and verified using DCLK1 siRNAs. Furthermore, miR‑1291 induced CDK inhibitors p21

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詳細情報 詳細情報について

  • CRID
    1360857593708368256
  • DOI
    10.3892/ijo.2022.5303
  • ISSN
    17912423
    10196439
  • PubMed
    34981812
  • 資料種別
    journal article
  • データソース種別
    • Crossref
    • KAKEN
    • OpenAIRE

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