Chromatic-aberration-free multispectral optical-resolution photoacoustic microscopy using reflective optics and a supercontinuum light source
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- Kazuyoshi Tachi
- National Defense Medical College
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- Takeshi Hirasawa
- National Defense Medical College
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- Shinpei Okawa
- National Defense Medical College
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- Akio Horiguchi
- National Defense Medical College
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- Keiichi Ito
- National Defense Medical College
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- Miya Ishihara
- National Defense Medical College
説明
<jats:p>A supercontinuum (SC) light source enables multispectral photoacoustic imaging at excitation wavelengths in the visible-to-near-infrared range. However, for such a broad optical wavelength range, chromatic aberration is non-negligible. We developed a multispectral optical-resolution photoacoustic microscopy (MS-OR-PAM) setup with a nanosecond pulsed SC light source and a reflective objective lens to avoid chromatic aberration. Chromatic aberrations generated by reflective and conventional objective lenses were compared, and the images acquired using the reflective objective were not affected by chromatic aberration. Hence, MS-OR-PAM with the reflective objective was used to distinguish red blood cells from melanoma cells via spectral subtraction processing.</jats:p>
収録刊行物
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- Applied Optics
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Applied Optics 60 (31), 9651-, 2021-10-21
Optica Publishing Group
