Ratiometric Fluorescent Lateral Flow Immunoassay for Point‐of‐Care Testing of Acute Myocardial Infarction
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- Jing Wang
- College of Chemical Engineering Zhejiang University of Technology Hangzhou 310014 PR China
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- Chenxing Jiang
- College of Chemical Engineering Zhejiang University of Technology Hangzhou 310014 PR China
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- Jiening Jin
- College of Chemical Engineering Zhejiang University of Technology Hangzhou 310014 PR China
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- Liang Huang
- College of Chemical Engineering Zhejiang University of Technology Hangzhou 310014 PR China
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- Wenbo Yu
- College of Veterinary Medicine China Agricultural University Beijing Key Laboratory of Detection Technology for Animal Derived Food Safety Beijing Laboratory for Food Quality and Safety Beijing 100193 PR China
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- Bin Su
- Institute of Analytical Chemistry Department of Chemistry Zhejiang University Hangzhou 310058 PR China
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- Jun Hu
- College of Chemical Engineering Zhejiang University of Technology Hangzhou 310014 PR China
説明
<jats:title>Abstract</jats:title><jats:p>We report the development of a highly sensitive ratiometric fluorescent lateral flow immunoassay (RFLFIA) strip for rapid and accurate detection of acute myocardial infarction biomarker, namely heart‐type fatty acid binding protein (H‐FABP). The RFLFIA strip works in terms of ratiometric change of fluorescence signal, arising from blending of fluorescence emitted by two composite nanostructures conjugated to capture and probe antibodies and inner filter effect of gold nanoparticles. In conjunction with using custom smartphone‐based analytical device and tonality analysis, quantitative detection of H‐FABP was achieved with a low limit of detection at 0.21 ng mL<jats:sup>−1</jats:sup>. The RFLFIA strip can generate a visually distinguishable green‐to‐red color change around the threshold concentration of H‐FABP (6.2 ng mL<jats:sup>−1</jats:sup>), thus allowing the semi‐quantitative diagnosis by the naked eye.</jats:p>
収録刊行物
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- Angewandte Chemie International Edition
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Angewandte Chemie International Edition 60 (23), 13042-13049, 2021-05-05
Wiley
