A switch in infected erythrocyte deformability at the maturation and blood circulation of Plasmodium falciparum transmission stages
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- Marta Tibúrcio
- Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanità, Rome, Italy;
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- Makhtar Niang
- Nanyang Technological University, School of Biological Sciences, Singapore;
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- Guillaume Deplaine
- Inserm–Université Pierre et Marie Curie, Unité Mixte de Recherche 945, Paris, France;
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- Sylvie Perrot
- Département de Parasitologie Mycologie, Unité d'Immunologie Moléculaire des Parasites, Centre National de la Recherche Scientifique (CNRS), URA 2581, Institut Pasteur, Paris, France;
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- Emmanuel Bischoff
- Département de Parasitologie Mycologie, Unité de Génétique et Génomique des Insectes Vecteurs, CNRS, URA 3012, Institut Pasteur, Paris, France;
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- Papa Alioune Ndour
- Inserm–Université Pierre et Marie Curie, Unité Mixte de Recherche 945, Paris, France;
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- Francesco Silvestrini
- Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanità, Rome, Italy;
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- Ayman Khattab
- Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland;
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- Geneviève Milon
- Département de Parasitologie Mycologie, Immunophysiologie et Parasitisme, Institut Pasteur, Paris, France;
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- Peter H. David
- Département de Parasitologie Mycologie, Unité d'Immunologie Moléculaire des Parasites, Centre National de la Recherche Scientifique (CNRS), URA 2581, Institut Pasteur, Paris, France;
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- Max Hardeman
- Department of Translational Physiology, Academic Medical Center, Amsterdam, The Netherlands; and
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- Kenneth D. Vernick
- Département de Parasitologie Mycologie, Unité de Génétique et Génomique des Insectes Vecteurs, CNRS, URA 3012, Institut Pasteur, Paris, France;
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- Robert W. Sauerwein
- Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
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- Peter R. Preiser
- Nanyang Technological University, School of Biological Sciences, Singapore;
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- Odile Mercereau-Puijalon
- Département de Parasitologie Mycologie, Unité d'Immunologie Moléculaire des Parasites, Centre National de la Recherche Scientifique (CNRS), URA 2581, Institut Pasteur, Paris, France;
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- Pierre Buffet
- Inserm–Université Pierre et Marie Curie, Unité Mixte de Recherche 945, Paris, France;
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- Pietro Alano
- Dipartimento di Malattie Infettive, Parassitarie e Immunomediate, Istituto Superiore di Sanità, Rome, Italy;
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- Catherine Lavazec
- Département de Parasitologie Mycologie, Unité d'Immunologie Moléculaire des Parasites, Centre National de la Recherche Scientifique (CNRS), URA 2581, Institut Pasteur, Paris, France;
抄録
<jats:title>Abstract</jats:title><jats:p>Achievement of malaria elimination requires development of novel strategies interfering with parasite transmission, including targeting the parasite sexual stages (gametocytes). The formation of Plasmodium falciparum gametocytes in the human host takes several days during which immature gametocyte-infected erythrocytes (GIEs) sequester in host tissues. Only mature stage GIEs circulate in the peripheral blood, available to uptake by the Anopheles vector. Mechanisms underlying GIE sequestration and release in circulation are virtually unknown. We show here that mature GIEs are more deformable than immature stages using ektacytometry and microsphiltration methods, and that a switch in cellular deformability in the transition from immature to mature gametocytes is accompanied by the deassociation of parasite-derived STEVOR proteins from the infected erythrocyte membrane. We hypothesize that mechanical retention contributes to sequestration of immature GIEs and that regained deformability of mature gametocytes is associated with their release in the bloodstream and ability to circulate. These processes are proposed to play a key role in P falciparum gametocyte development in the host and to represent novel and unconventional targets for interfering with parasite transmission.</jats:p>
収録刊行物
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- Blood
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Blood 119 (24), e172-e180, 2012-06-14
American Society of Hematology