Viral Culture Confirmed SARS-CoV-2 Subgenomic RNA Value as a Good Surrogate Marker of Infectivity

DOI Web Site 被引用文献1件
  • Marta Santos Bravo
    Microbiology Department, Hospital Clinic of Barcelona, Institute of Global Health of Barcelona (ISGlobal), Barcelona, Spain
  • Carla Berengua
    Microbiology Department, Hospital de la Santa Creu i Sant Pau, Universitat Autonòma de Barcelona, Barcelona, Spain
  • Pilar Marín
    Microbiology Department, Hospital de la Santa Creu i Sant Pau, Universitat Autonòma de Barcelona, Barcelona, Spain
  • Montserrat Esteban
    Microbiology Department, Hospital de la Santa Creu i Sant Pau, Universitat Autonòma de Barcelona, Barcelona, Spain
  • Cristina Rodriguez
    Microbiology Department, Hospital Clinic of Barcelona, Institute of Global Health of Barcelona (ISGlobal), Barcelona, Spain
  • Margarita del Cuerpo
    Microbiology Department, Hospital de la Santa Creu i Sant Pau, Universitat Autonòma de Barcelona, Barcelona, Spain
  • Elisenda Miró
    Microbiology Department, Hospital de la Santa Creu i Sant Pau, Universitat Autonòma de Barcelona, Barcelona, Spain
  • Genoveva Cuesta
    Microbiology Department, Hospital Clinic of Barcelona, Institute of Global Health of Barcelona (ISGlobal), Barcelona, Spain
  • Mar Mosquera
    Microbiology Department, Hospital Clinic of Barcelona, Institute of Global Health of Barcelona (ISGlobal), Barcelona, Spain
  • Sonsoles Sánchez-Palomino
    AIDS Research Group, Institut D’Investigacions Biomèdiques August Pi I Sunyer (IDIBAPS), Hospital Clinic, University of Barcelona, Barcelona, Spain
  • Jordi Vila
    Microbiology Department, Hospital Clinic of Barcelona, Institute of Global Health of Barcelona (ISGlobal), Barcelona, Spain
  • Núria Rabella
    Microbiology Department, Hospital de la Santa Creu i Sant Pau, Universitat Autonòma de Barcelona, Barcelona, Spain
  • María Ángeles Marcos
    Microbiology Department, Hospital Clinic of Barcelona, Institute of Global Health of Barcelona (ISGlobal), Barcelona, Spain
  • Angela M. Caliendo
    editor

この論文をさがす

説明

<jats:p> Determining SARS-CoV-2 viral infectivity is crucial for patient clinical assessment and isolation decisions. We assessed subgenomic RNA (sgRNA) as a surrogate marker of SARS-CoV-2 infectivity in SARS-CoV-2-positive reverse transcription PCR (RT-PCR) respiratory samples ( <jats:italic>n</jats:italic>  = 105) in comparison with viral culture as the reference standard for virus replication. sgRNA and viral isolation results were concordant in 99/105 cases (94%), indicating highly significant agreement between the two techniques (Cohen’s kappa coefficient 0.88, 95% confidence interval [CI] 0.78 to 0.97, <jats:italic>P</jats:italic>  < 0.001). sgRNA RT-PCR showed a sensitivity of 97% and a positive predictive value of 94% to detect replication-competent virus, further supporting sgRNA as a surrogate marker of SARS-CoV-2 infectivity. sgRNA RT-PCR is an accurate, rapid, and affordable technique that can overcome culture and cycle threshold ( <jats:italic> C <jats:sub>T</jats:sub> </jats:italic> ) value limitations and be routinely implemented in hospital laboratories to detect viral infectivity, which is essential for optimizing patient monitoring, the efficacy of treatments/vaccines, and work reincorporation policies, as well as for safely shortening isolation precautions. </jats:p>

収録刊行物

被引用文献 (1)*注記

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ