Encapsulation of hemoglobin in phospholipid vesicles
Description
<jats:p>Hemoglobin has been encapsulated in phospholipid vesicles by extrusion of hemoglobin/lipid mixtures through polycarbonate membranes. This technique avoids the use of organic solvents, sonication, and detergents which have proven deleterious to hemoglobin. The vesicles are homogeneous, with a mean size of 2400 Å as determined by photon correlation spectroscopy. The encapsulated hemoglobin binds oxygen reversibly and the vesicles are impermeable to ionic compounds. Hemoglobin encapsulated in egg phosphatidylcholine vesicles converts to methemoglobin within 2 days at 4°C. By contrast, when a mixture of dimyristoyl phosphatidylcholine, cholesterol and dicetyl phosphate is used there is no acceleration in methemoglobin formation, and the preparation is stable for at least 14 days at 4°C.</jats:p>
Journal
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- FEBS Letters
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FEBS Letters 153 (2), 285-288, 1983-03-21
Wiley
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Details 詳細情報について
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- CRID
- 1360857597729883392
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- ISSN
- 18733468
- 00145793
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- Data Source
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- Crossref