Application of a novel molecular method to age free‐living wild Bechstein's bats
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- Patrick G. R. Wright
- Bioscience College of Life and Environmental Sciences University of Exeter Exeter UK
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- Fiona Mathews
- College of Life Sciences University of Sussex Falmer UK
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- Henry Schofield
- The Vincent Wildlife Trust Ledbury Herefordshire UK
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- Colin Morris
- The Vincent Wildlife Trust Ledbury Herefordshire UK
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- Joe Burrage
- Exeter Medical School University of Exeter Exeter UK
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- Adam Smith
- Exeter Medical School University of Exeter Exeter UK
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- Emma L. Dempster
- Exeter Medical School University of Exeter Exeter UK
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- Patrick B. Hamilton
- Bioscience College of Life and Environmental Sciences University of Exeter Exeter UK
抄録
<jats:title>Abstract</jats:title><jats:p>The age profile of populations fundamentally affects their conservation status. Yet, age is frequently difficult to assess in wild animals. Here, we assessed the use of <jats:styled-content style="fixed-case">DNA</jats:styled-content> methylation of homologous genes to establish the age structure of a rare and elusive wild mammal: the Bechstein's bat (<jats:italic>Myotis bechsteinii</jats:italic>). We collected 62 wing punches from individuals whose ages were known as a result of a long‐term banding study. <jats:styled-content style="fixed-case">DNA</jats:styled-content> methylation was measured at seven CpG sites from three genes, which have previously shown age‐associated changes in humans and laboratory mice. All CpG sites from the tested genes showed a significant relationship between <jats:styled-content style="fixed-case">DNA</jats:styled-content> methylation and age, both individually and in combination (multiple linear regression <jats:italic>R</jats:italic><jats:sup>2</jats:sup> = 0.58, <jats:italic>p</jats:italic> < 0.001). Despite slight approximation around estimates, the approach is sufficiently precise to place animals into practically useful age cohorts. This method is of considerable practical benefit as it can reliably age individual bats. It is also much faster than traditional capture–mark–recapture techniques, with the potential to collect information on the age structure of an entire colony from a single sampling session to better inform conservation actions for Bechstein's bats. By identifying three genes where <jats:styled-content style="fixed-case">DNA</jats:styled-content> methylation correlates with age across distantly related species, this study also suggests that the technique can potentially be applied across a wide range of mammals.</jats:p>
収録刊行物
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- Molecular Ecology Resources
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Molecular Ecology Resources 18 (6), 1374-1380, 2018-07-27
Wiley