<i>Deianiraea</i>, an extracellular bacterium associated with the ciliate <i>Paramecium</i>, suggests an alternative scenario for the evolution of <i>Rickettsiales</i>
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- Michele Castelli
- Centro Romeo ed Enrica Invernizzi Ricerca Pediatrica, Dipartimento di Bioscienze, Università degli studi di Milano , Milan, Italy
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- Elena Sabaneyeva
- Department of Cytology and Histology, Faculty of Biology, Saint Petersburg State University , Saint Petersburg, Russia
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- Olivia Lanzoni
- Dipartimento di Biologia, Università di Pisa , Pisa, Italy
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- Natalia Lebedeva
- Centre of Core Facilities “Culture Collections of Microorganisms”, Saint Petersburg State University , Saint Petersburg, Russia
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- Anna Maria Floriano
- Dipartimento di Biologia e Biotecnologie, Università degli studi di Pavia , Pavia, Italy
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- Stefano Gaiarsa
- Dipartimento di Biologia e Biotecnologie, Università degli studi di Pavia , Pavia, Italy
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- Konstantin Benken
- Core Facility Center for Microscopy and Microanalysis, Saint Petersburg State University , Saint Petersburg, Russia
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- Letizia Modeo
- Dipartimento di Biologia, Università di Pisa , Pisa, Italy
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- Claudio Bandi
- Centro Romeo ed Enrica Invernizzi Ricerca Pediatrica, Dipartimento di Bioscienze, Università degli studi di Milano , Milan, Italy
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- Alexey Potekhin
- Department of Microbiology, Faculty of Biology, Saint Petersburg State University , Saint Petersburg, Russia
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- Davide Sassera
- Dipartimento di Biologia e Biotecnologie, Università degli studi di Pavia , Pavia, Italy
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- Giulio Petroni
- Dipartimento di Biologia, Università di Pisa , Pisa, Italy
説明
<jats:title>Abstract</jats:title> <jats:p>Rickettsiales are a lineage of obligate intracellular Alphaproteobacteria, encompassing important human pathogens, manipulators of host reproduction, and mutualists. Here we report the discovery of a novel Rickettsiales bacterium associated with Paramecium, displaying a unique extracellular lifestyle, including the ability to replicate outside host cells. Genomic analyses show that the bacterium possesses a higher capability to synthesise amino acids, compared to all investigated Rickettsiales. Considering these observations, phylogenetic and phylogenomic reconstructions, and re-evaluating the different means of interaction of Rickettsiales bacteria with eukaryotic cells, we propose an alternative scenario for the evolution of intracellularity in Rickettsiales. According to our reconstruction, the Rickettsiales ancestor would have been an extracellular and metabolically versatile bacterium, while obligate intracellularity would have evolved later, in parallel and independently, in different sub-lineages. The proposed new scenario could impact on the open debate on the lifestyle of the last common ancestor of mitochondria within Alphaproteobacteria.</jats:p>
収録刊行物
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- The ISME Journal
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The ISME Journal 13 (9), 2280-2294, 2019-05-09
Oxford University Press (OUP)
