A high-throughput cell- and virus-free assay shows reduced neutralization of SARS-CoV-2 variants by COVID-19 convalescent plasma

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  • Craig Fenwick
    Service of Immunology and Allergy, Department of Medicine, Lausanne University Hospital, University of Lausanne, Lausanne 1011, Switzerland.
  • Priscilla Turelli
    School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne 1015, Switzerland.
  • Céline Pellaton
    Service of Immunology and Allergy, Department of Medicine, Lausanne University Hospital, University of Lausanne, Lausanne 1011, Switzerland.
  • Alex Farina
    Service of Immunology and Allergy, Department of Medicine, Lausanne University Hospital, University of Lausanne, Lausanne 1011, Switzerland.
  • Jérémy Campos
    Service of Immunology and Allergy, Department of Medicine, Lausanne University Hospital, University of Lausanne, Lausanne 1011, Switzerland.
  • Charlène Raclot
    School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne 1015, Switzerland.
  • Florence Pojer
    School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne 1015, Switzerland.
  • Valeria Cagno
    Department of Microbiology and Molecular Medicine, University of Geneva, Geneva 1211, Switzerland.
  • Semira Gonseth Nusslé
    Centre for Primary Care and Public Health, University of Lausanne, Lausanne 1011, Switzerland.
  • Valerie D’Acremont
    Centre for Primary Care and Public Health, University of Lausanne, Lausanne 1011, Switzerland.
  • Jan Fehr
    Epidemiology, Biostatistics and Prevention Institute, University of Zurich, Zurich 8001, Switzerland.
  • Milo Puhan
    Epidemiology, Biostatistics and Prevention Institute, University of Zurich, Zurich 8001, Switzerland.
  • Giuseppe Pantaleo
    Service of Immunology and Allergy, Department of Medicine, Lausanne University Hospital, University of Lausanne, Lausanne 1011, Switzerland.
  • Didier Trono
    School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne 1015, Switzerland.

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<jats:p>A multiplexed virus-free surrogate neutralization assay measures antibody responses against spike protein mutations in SARS-CoV-2 variants.</jats:p>

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