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- Mykhaylo Usyk
- Department of Pediatrics, Albert Einstein College of Medicine, Bronx, New York, USA
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- Christine P. Zolnik
- Department of Pediatrics, Albert Einstein College of Medicine, Bronx, New York, USA
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- Hitesh Patel
- Department of Pathology, Montefiore Medical Center, Bronx, New York, USA
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- Michael H. Levi
- Department of Pathology, Montefiore Medical Center, Bronx, New York, USA
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- Robert D. Burk
- Department of Pediatrics, Albert Einstein College of Medicine, Bronx, New York, USA
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- Aaron P. Mitchell
- editor
説明
<jats:p>The mycobiome constitutes all the fungal organisms within an environment or biological niche. The fungi are eukaryotes, are extremely heterogeneous, and include yeasts and molds that colonize humans as part of the microbiome. In addition, fungi can also infect humans and cause disease. Characterization of the bacterial component of the microbiome was revolutionized by 16S rRNA gene fragment amplification, next-generation sequencing technologies, and bioinformatics pipelines. Characterization of the mycobiome has often not been included in microbiome studies because of limitations in amplification systems. This report revisited the selection of PCR primers that amplify the fungal ITS1 region. We have identified primers with superior identification of fungi present in the database. We have compared the new primer sets against those previously used in the literature and show a significant improvement in read count and taxon identification. These primers should facilitate the study of fungi in human physiology and disease states.</jats:p>
収録刊行物
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- mSphere
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mSphere 2 (6), 1-, 2017-12-27
American Society for Microbiology