Establishment of a reference panel of <i>Helicobacter pylori</i> strains for antimicrobial susceptibility testing
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- Kenji Yokota
- Working Group of the Reference Panel of Helicobacter pylori Strains for Antimicrobial Susceptibility Tests in Japanese Society for Helicobacter Research Tokyo Japan
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- Takako Osaki
- Working Group of the Reference Panel of Helicobacter pylori Strains for Antimicrobial Susceptibility Tests in Japanese Society for Helicobacter Research Tokyo Japan
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- Shunji Hayashi
- Working Group of the Reference Panel of Helicobacter pylori Strains for Antimicrobial Susceptibility Tests in Japanese Society for Helicobacter Research Tokyo Japan
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- Shin‐ichi Yokota
- Working Group of the Reference Panel of Helicobacter pylori Strains for Antimicrobial Susceptibility Tests in Japanese Society for Helicobacter Research Tokyo Japan
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- Hiroaki Takeuchi
- Working Group of the Reference Panel of Helicobacter pylori Strains for Antimicrobial Susceptibility Tests in Japanese Society for Helicobacter Research Tokyo Japan
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- Emiko Rimbara
- Department of Bacteriology II National Institute of Infectious Diseases (NIID) Musashimurayama Japan
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- Hinako Ojima
- Graduate School of Health Science Okayama University Okayama Japan
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- Toyotaka Sato
- Department of Microbiology Sapporo Medical University School of Medicine Sapporo Japan
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- Hideo Yonezawa
- Department of Infectious Diseases Kyorin University School of Medicine Mitaka Japan
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- Keigo Shibayama
- Department of Bacteriology Nagoya University Graduate School of Medicine Nagoya Japan
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- Kengo Tokunaga
- Department of General Medicine Kyorin University School of Medicine Mitaka Japan
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- Shigeru Kamiya
- Department of Infectious Diseases Kyorin University School of Medicine Mitaka Japan
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- Kazunari Murakami
- Department of Gastroenterology Faculty of Medicine Oita University Oita Japan
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- Mototsugu Kato
- Department of Gastroenterology National Hospital Organization National Hakodate Hospital Hakodate Japan
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- Toshiro Sugiyama
- Research Division of Molecular Target Therapeutics and Prevention of GI Cancer Hokkaido University Hospital Sapporo Japan
Abstract
<jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>Eradication treatment for <jats:italic>Helicobacter pylori</jats:italic> gastritis is covered by national health insurance since 2013 in Japan. However, eradication failure due to the increase of antimicrobial resistance has become a serious problem. The present study aims to establish a reference panel of Japanese <jats:italic>H. pylori</jats:italic> strains for antimicrobial susceptibility testing.</jats:p></jats:sec><jats:sec><jats:title>Method</jats:title><jats:p>A total of 28 strains were collected from 4 medical facilities in Japan. Antimicrobial susceptibility tests (ASTs) to clarithromycin (CLR), amoxicillin (AMX), and metronidazole (MNZ), were used to select standard reference strains. Complete genome sequences were also determined.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Three <jats:italic>H. pylori</jats:italic> strains (JSHR3, JSHR6 and JSHR31) were selected as standard reference strains by the Japanese Society for <jats:italic>Helicobacter</jats:italic> Research (JSHR). The minimum inhibitory concentrations (MICs) of the antibiotics against these 3 strains by agar dilution method with Brucella‐based horse‐serum‐containing agar medium were as follows: JSHR3 (CLR 16 μg/ml, AMX 0.032 μg/ml and MNZ 4 μg/ml), JSHR6 (CLR 0.016 μg/ml, AMX 0.032 μg/ml and MNZ 4 μg/ml), and JSHR31 (CLR 16 μg/ml, AMX 1 μg/ml and MNZ 64 μg/ml).</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>A reference panel of <jats:italic>H. pylori</jats:italic> JSHR strains was established. The panel consisted of JSHR6, which was antibiotic‐susceptible, JSHR3, which was CLR‐resistant, and JSHR31, which was multi‐resistant. This reference panel will be essential for standardized ASTs before the optimal drugs are selected for eradication treatment.</jats:p></jats:sec>
Journal
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- Helicobacter
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Helicobacter 27 (3), 2022-03-07
Wiley
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Details 詳細情報について
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- CRID
- 1360861289648796160
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- ISSN
- 15235378
- 10834389
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- Data Source
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- Crossref
- KAKEN