Demonstration of two distinct antigenic determinants on hepatitis B e antigen by monoclonal antibodies.
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- M Imai
- Immunology Division, Jichi Medical School; the Hepatitis Division, the Tokyo Metropolitan Institute of Medical Science, University of Tokyo ,
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- M Nomura
- Immunology Division, Jichi Medical School; the Hepatitis Division, the Tokyo Metropolitan Institute of Medical Science, University of Tokyo ,
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- T Gotanda
- Immunology Division, Jichi Medical School; the Hepatitis Division, the Tokyo Metropolitan Institute of Medical Science, University of Tokyo ,
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- T Sano
- Immunology Division, Jichi Medical School; the Hepatitis Division, the Tokyo Metropolitan Institute of Medical Science, University of Tokyo ,
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- K Tachibana
- Immunology Division, Jichi Medical School; the Hepatitis Division, the Tokyo Metropolitan Institute of Medical Science, University of Tokyo ,
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- H Miyamoto
- Immunology Division, Jichi Medical School; the Hepatitis Division, the Tokyo Metropolitan Institute of Medical Science, University of Tokyo ,
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- K Takahashi
- Immunology Division, Jichi Medical School; the Hepatitis Division, the Tokyo Metropolitan Institute of Medical Science, University of Tokyo ,
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- S Toyama
- Immunology Division, Jichi Medical School; the Hepatitis Division, the Tokyo Metropolitan Institute of Medical Science, University of Tokyo ,
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- Y Miyakawa
- Immunology Division, Jichi Medical School; the Hepatitis Division, the Tokyo Metropolitan Institute of Medical Science, University of Tokyo ,
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- M Mayumi
- Immunology Division, Jichi Medical School; the Hepatitis Division, the Tokyo Metropolitan Institute of Medical Science, University of Tokyo ,
書誌事項
- 公開日
- 1982-01-01
- 権利情報
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- https://academic.oup.com/pages/standard-publication-reuse-rights
- DOI
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- 10.4049/jimmunol.128.1.69
- 公開者
- Oxford University Press (OUP)
この論文をさがす
説明
<jats:title>Abstract</jats:title> <jats:p>Mice were immunized against hepatitis B e antigen (HBeAg) isolated from sera of asymptomatic carriers of hepatitis B virus. Their spleen cells were fused with mouse myeloma (NS-1) cells, and 5 clones of hybridoma cells secreting antibody against HBeAg (anti-HBe) were isolated. For the production of anti-HBe in large scale, cells were cultivated both in vitro and in the peritoneal cavity of ascitic mice. Although monoclonal antibodies produced by these clones showed a strong reactivity of anti-HBe in hemagglutination tests, individual monoclonal anti-HBe did not reveal any precipitin line in immunodiffusion. When 2 of the 5 monoclonal antibodies were mixed together, however, some combinations showed a precipitin line against HBeAg, whereas others did not. Utilizing solid-phase radioimmunoassay involving a number of combinations of monoclonal antibodies used for solid-phase and radiolabeling, the 5 antibodies were classified into 2 groups. Three of the anti-HBe antibodies were found to be directed to 1 determinant of HBeAg (determinant a); the remaining 2 to the other determinant (determinant b). Determinants a and b were detected on HBeAg in the serum, as well as on the polypeptide of 19,000 daltons (P19) derived from the nucleocapsid of hepatitis B virus. Monoclonal anti-HBe antibodies with different specificities may provide useful tools in delineating the antigenic structure of HBeAg and also in evaluating immune responses of the host directed to its subdeterminants.</jats:p>
収録刊行物
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- The Journal of Immunology
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The Journal of Immunology 128 (1), 69-72, 1982-01-01
Oxford University Press (OUP)