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- Chun So
- Department of Meiosis, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.
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- Katerina Menelaou
- Department of Meiosis, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.
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- Julia Uraji
- Department of Meiosis, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.
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- Katarina Harasimov
- Department of Meiosis, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.
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- Anna M. Steyer
- Electron Microscopy Core Unit, Department of Neurogenetics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.
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- K. Bianka Seres
- Department of Meiosis, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.
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- Jonas Bucevičius
- Chromatin Labeling and Imaging Group, Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.
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- Gražvydas Lukinavičius
- Chromatin Labeling and Imaging Group, Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.
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- Wiebke Möbius
- Electron Microscopy Core Unit, Department of Neurogenetics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.
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- Claus Sibold
- Fertility Center Berlin, Berlin, Germany.
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- Andreas Tandler-Schneider
- Fertility Center Berlin, Berlin, Germany.
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- Heike Eckel
- Kinderwunschzentrum Göttingen, Göttingen, Germany.
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- Rüdiger Moltrecht
- Kinderwunschzentrum Göttingen, Göttingen, Germany.
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- Martyn Blayney
- Bourn Hall Clinic, Cambridge, UK.
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- Kay Elder
- Bourn Hall Clinic, Cambridge, UK.
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- Melina Schuh
- Department of Meiosis, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.
抄録
<jats:p>Human oocytes are prone to assembling meiotic spindles with unstable poles, which can favor aneuploidy in human eggs. The underlying causes of spindle instability are unknown. We found that NUMA (nuclear mitotic apparatus protein)–mediated clustering of microtubule minus ends focused the spindle poles in human, bovine, and porcine oocytes and in mouse oocytes depleted of acentriolar microtubule-organizing centers (aMTOCs). However, unlike human oocytes, bovine, porcine, and aMTOC-free mouse oocytes have stable spindles. We identified the molecular motor KIFC1 (kinesin superfamily protein C1) as a spindle-stabilizing protein that is deficient in human oocytes. Depletion of KIFC1 recapitulated spindle instability in bovine and aMTOC-free mouse oocytes, and the introduction of exogenous KIFC1 rescued spindle instability in human oocytes. Thus, the deficiency of KIFC1 contributes to spindle instability in human oocytes.</jats:p>
収録刊行物
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- Science
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Science 375 (6581), 2022-02-11
American Association for the Advancement of Science (AAAS)