Rewiring <i>Yarrowia lipolytica</i> toward triacetic acid lactone for materials generation
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- Kelly A. Markham
- McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712;
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- Claire M. Palmer
- Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712
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- Malgorzata Chwatko
- McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712;
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- James M. Wagner
- McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712;
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- Clare Murray
- McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712;
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- Sofia Vazquez
- McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712;
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- Arvind Swaminathan
- McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712;
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- Ishani Chakravarty
- McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712;
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- Nathaniel A. Lynd
- McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712;
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- Hal S. Alper
- McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712;
説明
<jats:title>Significance</jats:title> <jats:p> Polyketides are important molecules for both their bioactive traits and their potential as chemical building blocks. However, production of these molecules through chemistry and biocatalysts is restricted in yield and titer. Here, we demonstrate that the nonconventional yeast <jats:italic>Yarrowia lipolytica</jats:italic> can serve as a potent host for such production. This work provides a comprehensive evaluation of three separate pathways toward acetyl–CoA and malonyl–CoA in this host, enabling high-titer production of triacetic acid lactone. Beyond achieving unprecedented titers and appreciable yields, this production capacity allows for both purification from fermentation broth and conversion into a material using simple reaction conditions. </jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 115 (9), 2096-2101, 2018-02-12
Proceedings of the National Academy of Sciences