T6SS secretes an LPS-binding effector to recruit OMVs for exploitative competition and horizontal gene transfer
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- Changfu Li
- State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University , Yangling, Shaanxi, China
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- Lingfang Zhu
- State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University , Yangling, Shaanxi, China
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- Dandan Wang
- Department of Biochemistry and Molecular Biology, College of Life Sciences, Northwest A&F University , Yangling, Shaanxi, China
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- Zhiyan Wei
- State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University , Yangling, Shaanxi, China
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- Xinwei Hao
- State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University , Yangling, Shaanxi, China
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- Zhuo Wang
- Department of Biochemistry and Molecular Biology, College of Life Sciences, Northwest A&F University , Yangling, Shaanxi, China
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- Tengfei Li
- College of Grassland and Agriculture, Northwest A&F University , Yangling, Shaanxi, China
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- Lei Zhang
- State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University , Yangling, Shaanxi, China
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- Zhiqiang Lu
- Department of Entomology, College of Plant Protection, Northwest A&F University , Yangling, Shaanxi, China
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- Mingxiu Long
- College of Grassland and Agriculture, Northwest A&F University , Yangling, Shaanxi, China
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- Yao Wang
- Department of Biochemistry and Molecular Biology, College of Life Sciences, Northwest A&F University , Yangling, Shaanxi, China
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- Gehong Wei
- State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University , Yangling, Shaanxi, China
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- Xihui Shen
- State Key Laboratory of Crop Stress Biology for Arid Areas, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University , Yangling, Shaanxi, China
書誌事項
- 公開日
- 2021-08-25
- 権利情報
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- https://creativecommons.org/licenses/by/4.0/
- https://creativecommons.org/licenses/by/4.0
- https://creativecommons.org/licenses/by/4.0
- DOI
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- 10.1038/s41396-021-01093-8
- 公開者
- Oxford University Press (OUP)
この論文をさがす
説明
<jats:title>Abstract</jats:title> <jats:p>Outer membrane vesicles (OMVs) can function as nanoscale vectors that mediate bacterial interactions in microbial communities. How bacteria recognize and recruit OMVs inter-specifically remains largely unknown, thus limiting our understanding of the complex physiological and ecological roles of OMVs. Here, we report a ligand-receptor interaction-based OMV recruitment mechanism, consisting of a type VI secretion system (T6SS)-secreted lipopolysaccharide (LPS)-binding effector TeoL and the outer membrane receptors CubA and CstR. We demonstrated that Cupriavidus necator T6SS1 secretes TeoL to preferentially associate with OMVs in the extracellular milieu through interactions with LPS, one of the most abundant components of OMVs. TeoL associated with OMVs can further bind outer membrane receptors CubA and CstR, which tethers OMVs to the recipient cells and allows cargo to be delivered. The LPS-mediated mechanism enables bacterial cells to recruit OMVs derived from different species, and confers advantages to bacterial cells in iron acquisition, interbacterial competition, and horizontal gene transfer (HGT). Moreover, our findings provide multiple new perspectives on T6SS functionality in the context of bacterial competition and HGT, through the recruitment of OMVs.</jats:p>
収録刊行物
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- The ISME Journal
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The ISME Journal 16 (2), 500-510, 2021-08-25
Oxford University Press (OUP)
