Chemiluminescent Assay for Glycerol in Wine Using Flow‐Injection

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<jats:title>ABSTRACT</jats:title><jats:p>A flow‐injection method was developed for the enzymatic determination of glycerol in wines using a reactor containing immobilized glycerokinase and glycerol‐3′‐phosphate oxidase. Hydrogen peroxide from the reaction was monitored by chemiluminescence after reaction with an alkaline reagent containing luminol and hexacyanoferrate (III). Calibration curves were linear between 25 mg/L and 250 mg/L of glycerol for injected volume 29 μL. The method gave satisfactory results compared with a gas‐chromatographic method. No sample pre‐treatment was necessary and a sampling frequency of 30/hr could be achieved.</jats:p>

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