IL-2 and IFN-gamma are two necessary lymphokines in the development of cytolytic T cells.

DOI PDF 被引用文献8件
  • E Maraskovsky
    Walter and Eliza Hall Institute of Medical Research, P.O. Royal Melbourne Hospital , Victoria 3050 ,
  • W F Chen
    Walter and Eliza Hall Institute of Medical Research, P.O. Royal Melbourne Hospital , Victoria 3050 ,
  • K Shortman
    Walter and Eliza Hall Institute of Medical Research, P.O. Royal Melbourne Hospital , Victoria 3050 ,

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公開日
1989-08
権利情報
  • https://academic.oup.com/pages/standard-publication-reuse-rights
DOI
  • 10.4049/jimmunol.143.4.1210
公開者
Oxford University Press (OUP)

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<jats:title>Abstract</jats:title> <jats:p>A filler cell-free limiting-dilution microculture system has been developed for the expansion and differentiation of a high proportion of single CD4-CD8+ T cells into cytolytic T cell (CTL) clones. The stimulus used was PMA together with the calcium ionophore ionomycin. The growth and differentiation factors were rIL-2, together with either a Con A-stimulated spleen cell supernatant (CAS) or rIFN-gamma. CTL activity was monitored by an autoradiographic 111In-release assay. With CAS and rIL-2 present, 50% of all potential precursors (CTL-p) produced cytolytic clones. Substitution of rIFN-gamma for CAS gave a similar efficiency with up to 42% of CTL-p producing cytolytic clones. rIL-2 alone allowed only a small proportion (6%) of CD4-CD8+ T cells to become cytolytic clones. Addition of rIL-2 and rIFN-gamma at various stages of the culture demonstrated that IL-2 was required throughout, but exogenous IFN-gamma was required only during the early stages. It is concluded that for at least 40% of all CTL-p, the lymphokines IL-2 and IFN-gamma are essential and act in synergy to induce proliferation and differentiation into CTL.</jats:p>

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