Biocompatibility and hemocompatibility of efficiently decellularized whole porcine kidney for tissue engineering

  • Kamal Hany Hussein
    Department of Animal Surgery, College of Veterinary Medicine Assiut University Assiut 71515 Egypt
  • Tarek Saleh
    Stem Cell Institute, Kangwon National University Chuncheon Gangwon 200‐701 Republic of Korea
  • Ebtehal Ahmed
    Stem Cell Institute, Kangwon National University Chuncheon Gangwon 200‐701 Republic of Korea
  • Ho‐Hyun Kwak
    Stem Cell Institute, Kangwon National University Chuncheon Gangwon 200‐701 Republic of Korea
  • Kyung‐Mee Park
    Department of Surgery, College of Veterinary Medicine Chungbuk National University Cheongju Chungbuk Republic of Korea
  • Se‐Ran Yang
    Stem Cell Institute, Kangwon National University Chuncheon Gangwon 200‐701 Republic of Korea
  • Byung‐Jae Kang
    Department of Surgery, College of Veterinary Medicine Kangwon National University Chuncheon Gangwon 200‐701 Republic of Korea
  • Ki‐Young Choi
    Department of Controlled Agriculture Kangwon National University Chuncheon Gangwon 200‐701 Republic of Korea
  • Kyung‐Sun Kang
    Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University Seoul 08826 Republic of Korea
  • Heung‐Myong Woo
    Stem Cell Institute, Kangwon National University Chuncheon Gangwon 200‐701 Republic of Korea

抄録

<jats:title>Abstract</jats:title><jats:p>Whole kidney decellularization is a promising approach in regenerative medicine for engineering a functional organ. The reaction of the potential host depends on the biocompatibility of these decellularized constructs. Despite the proven ability of decellularized kidney scaffolds to guide cell attachment and growth, little is known about biocompatibility and hemocompatibility of these scaffolds. Our aim is to prepare decellularized kidneys of a clinically relevant size and evaluate its biocompatibility and hemocompatibility. Porcine kidneys were cannulated via the renal artery, and then perfused with 0.1% sodium dodecyl sulfate solution. Hematoxylin and eosin as well as DAPI staining confirmed cellular clearance from native kidneys in addition to preservation of the microstructure. SEM confirmed the absence of any cellular content within the scaffold, which is maintained in a well‐organized 3D architecture. Decellularized kidneys retained the intact renal vasculature upon examination with contrast radiography. The essential structural extracellular matrix molecules were well‐preserved. Scaffolds were susceptible to enzymatic degradation upon collagenase treatment. Scaffolds showed a good hemocompatibility when exposed to porcine blood. Decellularization was efficient to remove 97.7% of DNA from native kidneys in addition to the immunogenic and pathogenic antigens. Scaffolds did not induce the human immune response <jats:italic>in vitro</jats:italic>. Decellularized kidneys were non‐cytotoxic to pig kidney cells (PKs). PKs were able to grow and proliferate within the decellularized renal scaffolds with maintaining a higher function than cells grown as monolayers. Thus, we have developed a rapid decellularization technique for generating biocompatible kidney scaffolds that represents a step toward development of a transplantable organ. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2034–2047, 2018.</jats:p>

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