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- P T Mehrotra
- Laboratory of Cellular Immunology, Food and Drug Administration, Rockville, MD 20852, USA.
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- A J Grant
- Laboratory of Cellular Immunology, Food and Drug Administration, Rockville, MD 20852, USA.
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- J P Siegel
- Laboratory of Cellular Immunology, Food and Drug Administration, Rockville, MD 20852, USA.
説明
<jats:title>Abstract</jats:title> <jats:p>We have previously demonstrated that human rIL-12 alone can augment the development of cytotoxic activity in stimulated CD8+ T cells. The present study was undertaken to examine the interactions of rIL-7 and rIL-12 on human peripheral blood T cell activation and CTL differentiation. Purified T lymphocytes were pulsed overnight with immobilized alpha-CD3 and then cultured for 3 additional days with IL-7 and/or IL-12. The combination of IL-7 and IL-12 synergistically enhanced the proliferation of either fresh CD3+ T cells or an IL-2-dependent CD4+ T cell line, Kit-225-K6. This synergy was seen on both subsets of T cells; however, CD8+ T cells were usually more responsive to IL-7 and IL-12 at lower concentrations than were CD4+ T cells. Furthermore, these cytokines additively/synergistically augmented the cytotoxic activity of CD8+ T cells. Abs to IL-2 and IL-2R alpha blocked the synergistic effect on proliferation of CD4+ T cells, but had a minimal effect on the synergistic response of the proliferative and cytotoxic activity of CD8+ T cells. Examination of the effects of IL-7 and IL-12 on the expression of IL-12 receptor on T cells revealed an increase in the subunit of IL-12R by IL-7 as determined by flow cytometric analysis. We analyzed the effects on IFN-gamma production by CD8+ T cells and found that IL-7 alone did not induce detectable levels of IFN-gamma production but together with IL-12 it synergistically enhanced the production of IFN-gamma. We also found that IFN-gamma was probably not required for enhanced CTL activity of CD8+ T cells, because Ab to human IFN-gamma did not block additive/synergistic effects of either cytokine. The synergistic stimulatory activity of IL-7 and IL-12 may be of significance in vivo and may provide an alternative mechanism of stimulating T cells for use in immunotherapy.</jats:p>
収録刊行物
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- The Journal of Immunology
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The Journal of Immunology 154 (10), 5093-5102, 1995-05-15
The American Association of Immunologists
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詳細情報 詳細情報について
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- CRID
- 1360861295373382016
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- ISSN
- 15506606
- 00221767
- http://id.crossref.org/issn/00221767
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- データソース種別
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- Crossref