The Cdc25/Ras/cAMP-dependent protein kinase A signaling pathway regulates proline utilization in wine yeast <i>Saccharomyces cerevisiae</i> under a wine fermentation model
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- Akira Nishimura
- Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology , 8916-5 Takayama-cho, Ikoma, Nara, Japan
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- Kazuki Ichikawa
- Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology , 8916-5 Takayama-cho, Ikoma, Nara, Japan
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- Hayate Nakazawa
- Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology , 8916-5 Takayama-cho, Ikoma, Nara, Japan
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- Ryoya Tanahashi
- Division for Research Strategy, Institute for Research Initiatives, Nara Institute of Science and Technology , 8916-5 Takayama-cho, Ikoma, Nara, Japan
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- Fumika Morita
- Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology , 8916-5 Takayama-cho, Ikoma, Nara, Japan
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- Irnayuli Sitepu
- Phaff Yeast Culture Collection, Department of Food Science and Technology, University of California Davis , One Shields Ave, Davis, CA, USA
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- Kyria Boundy-Mills
- Phaff Yeast Culture Collection, Department of Food Science and Technology, University of California Davis , One Shields Ave, Davis, CA, USA
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- Glen Fox
- Department of Food Science and Technology, University of California Davis , One Shields Ave, Davis, CA, USA
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- Hiroshi Takagi
- Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology , 8916-5 Takayama-cho, Ikoma, Nara, Japan
説明
<jats:title>ABSTRACT</jats:title> <jats:p>Proline is a predominant amino acid in grape must, but it is poorly utilized by the yeast Saccharomyces cerevisiae in wine-making processes. This sometimes leads to a nitrogen deficiency during fermentation and proline accumulation in wine. In this study, we clarified that a glucose response is involved in an inhibitory mechanism of proline utilization in yeast. Our genetic screen showed that strains with a loss-of-function mutation on the CDC25 gene can utilize proline even under fermentation conditions. Cdc25 is a regulator of the glucose response consisting of the Ras/cAMP-dependent protein kinase A (PKA) pathway. Moreover, we found that activation of the Ras/PKA pathway is necessary for the inhibitory mechanism of proline utilization. The present data revealed that crosstalk exists between the carbon and proline metabolisms. Our study could hold promise for the development of wine yeast strains that can efficiently assimilate proline during the fermentation processes.</jats:p>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 86 (9), 1318-1326, 2022-06-24
Oxford University Press (OUP)
