説明
<jats:title>Abstract</jats:title> <jats:p>The polymerase chain reaction makes possible a new method for engineering genes and is useful for both mutagenesis and recombination of sequences. This approach has significant advantages over current techniques because it is fast, straightforward, and not limited by the need for restriction sites. The development of recombinant DNA technology can be divided into three generations. The first generation approaches take advantage of natural recombination mechanisms in vivo to map and study genes. Into this category would fall classical genetic linkage analysis, and the use of mechanisms for homologous recombination between phages, for example, to generate sets of random recombinants between related genes. The second generation began with the use of restriction endonucleases and DNA ligase to specifically take genes apart, and put them back together in new combinations in vitro. It was the advent of this technology that made the field of molecular genetics what it is today.</jats:p>
収録刊行物
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- Directed Mutagenesis
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Directed Mutagenesis 217-247, 1991-06-27
Oxford University PressOxford