Relationship between frame rates and subpopulation structure of bovine sperm divided by their motility analyzed by a computer‐assisted sperm analysis system

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  • Chihiro Kanno
    Laboratory of Clinical Veterinary Medicine for Large Animal, Division of Veterinary Medicine, School of Veterinary Medicine Kitasato University Towada Japan
  • Kang Sun‐Sik
    Hanwoo Research Institute National Institute of Animal Science of Korea Pyeongchang Korea
  • Kentaro Q. Sakamoto
    Department of Marine Bioscience, Atmosphere and Ocean Research Institute The University of Tokyo Kashiwa Japan
  • Yojiro Yanagawa
    Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine Hokkaido University Sapporo Japan
  • Seiji Katagiri
    Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine Hokkaido University Sapporo Japan
  • Masashi Nagano
    Laboratory of Animal Reproduction, Department of Animal Science, School of Veterinary Medicine Kitasato University Towada Japan

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<jats:title>Abstract</jats:title><jats:p>The authors investigated the relationship between frame rates and subpopulation structure of bovine sperm divided by their motility analyzed by a computer‐assisted sperm analysis (CASA). Kinematic parameters of bovine sperm incubated in Brackett & Oliphant medium with and without calcium ionophore for 0, 1, 5, 10, 15, 20, 25, and 30 min were evaluated by a CASA at 150 frames per second (fps) and analyzed structure of sperm motility subpopulation by cluster analysis. Then, we converted CASA data at 150 fps to 75, 50, and 30 fps and evaluated the structures of sperm motility subpopulation at different fps in each sperm by a discriminant analysis. As the results, the structure of sperm motility subpopulation was affected by frame rate. Sperm were divided into six clusters at 150, 75, and 50 fps; on the other hand, there were five clusters at 30 fps. Straight‐line velocity was similar at all frame rates. However, as the frame rate became higher, curvilinear velocity and beat cross frequency of sperm head increased significantly, whereas lateral sperm head displacement decreased significantly. In conclusion, higher frame rate at 150 fps is recommended to capture the trajectory of sperm accurately by CASA in the present study.</jats:p>

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