Temperature Dependence of the Beating Frequency of hiPSC-CMs Using a MEMS Force Sensor

  • Ryota Ikegami
    Department of Intelligent Robotics, Faculty of Engineering, Toyama Prefectural University, Imizu 939-0398, Japan
  • Takuya Tsukagoshi
    Department of Intelligent Robotics, Faculty of Engineering, Toyama Prefectural University, Imizu 939-0398, Japan
  • Kenei Matsudaira
    Department of Mechano-Informatics, Graduate School of Information Science and Technology, The University of Tokyo, Tokyo 113-8654, Japan
  • Kayoko Hirayama Shoji
    Department of Mechano-Informatics, Graduate School of Information Science and Technology, The University of Tokyo, Tokyo 113-8654, Japan
  • Hidetoshi Takahashi
    Department of Mechanical Engineering, Faculty of Science and Technology, Keio University, Yokohama 223-8522, Japan
  • Thanh-Vinh Nguyen
    Sensing System Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8564, Japan
  • Takumi Tamamoto
    Department of Intelligent Mechanical Engineering, Faculty of Engineering, Fukuoka Institute of Technology, Fukuoka 811-0295, Japan
  • Kentaro Noda
    Department of Intelligent Robotics, Faculty of Engineering, Toyama Prefectural University, Imizu 939-0398, Japan
  • Ken’ichi Koyanagi
    Department of Intelligent Robotics, Faculty of Engineering, Toyama Prefectural University, Imizu 939-0398, Japan
  • Toru Oshima
    Department of Intelligent Robotics, Faculty of Engineering, Toyama Prefectural University, Imizu 939-0398, Japan
  • Isao Shimoyama
    Toyama Prefectural University, Imizu 939-0398, Japan

説明

<jats:p>It is expected that human iPS cell-derived cardiomyocytes (hiPSC-CMs) can be used to treat serious heart diseases. However, the properties and functions of human adult cardiomyocytes and hiPSC-CMs, including cell maturation, differ. In this study, we focused on the temperature dependence of hiPSC-CMs by integrating the temperature regulation system into our sensor platform, which can directly and quantitatively measure their mechanical motion. We measured the beating frequency of hiPSC-CMs at different environmental temperatures and found that the beating frequency increased as the temperature increased. Although the rate at which the beating frequency increased with temperature varied, the temperature at which the beating stopped was relatively stable at approximately 20 °C. The stopping of beating at this temperature was stable, even in immature hiPSC-CMs, and was considered to be a primitive property of cardiomyocytes.</jats:p>

収録刊行物

  • Sensors

    Sensors 23 (7), 3370-, 2023-03-23

    MDPI AG

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