Temperature Dependence of the Beating Frequency of hiPSC-CMs Using a MEMS Force Sensor
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- Ryota Ikegami
- Department of Intelligent Robotics, Faculty of Engineering, Toyama Prefectural University, Imizu 939-0398, Japan
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- Takuya Tsukagoshi
- Department of Intelligent Robotics, Faculty of Engineering, Toyama Prefectural University, Imizu 939-0398, Japan
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- Kenei Matsudaira
- Department of Mechano-Informatics, Graduate School of Information Science and Technology, The University of Tokyo, Tokyo 113-8654, Japan
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- Kayoko Hirayama Shoji
- Department of Mechano-Informatics, Graduate School of Information Science and Technology, The University of Tokyo, Tokyo 113-8654, Japan
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- Hidetoshi Takahashi
- Department of Mechanical Engineering, Faculty of Science and Technology, Keio University, Yokohama 223-8522, Japan
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- Thanh-Vinh Nguyen
- Sensing System Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8564, Japan
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- Takumi Tamamoto
- Department of Intelligent Mechanical Engineering, Faculty of Engineering, Fukuoka Institute of Technology, Fukuoka 811-0295, Japan
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- Kentaro Noda
- Department of Intelligent Robotics, Faculty of Engineering, Toyama Prefectural University, Imizu 939-0398, Japan
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- Ken’ichi Koyanagi
- Department of Intelligent Robotics, Faculty of Engineering, Toyama Prefectural University, Imizu 939-0398, Japan
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- Toru Oshima
- Department of Intelligent Robotics, Faculty of Engineering, Toyama Prefectural University, Imizu 939-0398, Japan
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- Isao Shimoyama
- Toyama Prefectural University, Imizu 939-0398, Japan
説明
<jats:p>It is expected that human iPS cell-derived cardiomyocytes (hiPSC-CMs) can be used to treat serious heart diseases. However, the properties and functions of human adult cardiomyocytes and hiPSC-CMs, including cell maturation, differ. In this study, we focused on the temperature dependence of hiPSC-CMs by integrating the temperature regulation system into our sensor platform, which can directly and quantitatively measure their mechanical motion. We measured the beating frequency of hiPSC-CMs at different environmental temperatures and found that the beating frequency increased as the temperature increased. Although the rate at which the beating frequency increased with temperature varied, the temperature at which the beating stopped was relatively stable at approximately 20 °C. The stopping of beating at this temperature was stable, even in immature hiPSC-CMs, and was considered to be a primitive property of cardiomyocytes.</jats:p>
収録刊行物
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- Sensors
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Sensors 23 (7), 3370-, 2023-03-23
MDPI AG
- Tweet
キーワード
- Adult
- Chemical technology
- Induced Pluripotent Stem Cells
- Temperature
- cardiomyocytes
- Cell Differentiation
- TP1-1185
- Micro-Electrical-Mechanical Systems
- piezoresistive cantilever; cardiomyocytes; temperature dependence
- Article
- piezoresistive cantilever
- Humans
- Myocytes, Cardiac
- temperature dependence
- Cells, Cultured
詳細情報 詳細情報について
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- CRID
- 1360861705586716160
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- ISSN
- 14248220
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- PubMed
- 37050430
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- 資料種別
- journal article
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- データソース種別
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- Crossref
- KAKEN
- OpenAIRE