A fish herpesvirus highlights functional diversities among Zα domains related to phase separation induction and A-to-Z conversion

DOI PDF 参考文献80件 オープンアクセス
  • Mamadou Amadou Diallo
    Department of Infectious and Parasitic Diseases, Immunology-Vaccinology, University of Liège , Liège  B-4000, Belgium
  • Sébastien Pirotte
    Department of Infectious and Parasitic Diseases, Immunology-Vaccinology, University of Liège , Liège  B-4000, Belgium
  • Yunlong Hu
    Department of Infectious and Parasitic Diseases, Immunology-Vaccinology, University of Liège , Liège  B-4000, Belgium
  • Léa Morvan
    Department of Infectious and Parasitic Diseases, Immunology-Vaccinology, University of Liège , Liège  B-4000, Belgium
  • Krzysztof Rakus
    Department of Infectious and Parasitic Diseases, Immunology-Vaccinology, University of Liège , Liège  B-4000, Belgium
  • Nicolás M Suárez
    MRC-University of Glasgow Centre for Virus Research , Glasgow  G61 1QH, UK
  • Lee PoTsang
    Department of Infectious and Parasitic Diseases, Immunology-Vaccinology, University of Liège , Liège  B-4000, Belgium
  • Hisao Saneyoshi
    Department of Medical Sciences, Division of Chemistry, University of Miyazaki , Miyazaki  889-1692, Japan
  • Yan Xu
    Department of Medical Sciences, Division of Chemistry, University of Miyazaki , Miyazaki  889-1692, Japan
  • Andrew J Davison
    MRC-University of Glasgow Centre for Virus Research , Glasgow  G61 1QH, UK
  • Peter Tompa
    VIB-VUB Center for Structural Biology, Vrije Universiteit Brussel , Brussel  B-1050, Belgium
  • Joel L Sussman
    Department of Chemical and Structural Biology, Weizmann Institute of Science , Rehovot  7610001, Israel
  • Alain Vanderplasschen
    Department of Infectious and Parasitic Diseases, Immunology-Vaccinology, University of Liège , Liège  B-4000, Belgium

抄録

<jats:title>Abstract</jats:title> <jats:p>Zalpha (Zα) domains bind to left-handed Z-DNA and Z-RNA. The Zα domain protein family includes cellular (ADAR1, ZBP1 and PKZ) and viral (vaccinia virus E3 and cyprinid herpesvirus 3 (CyHV-3) ORF112) proteins. We studied CyHV-3 ORF112, which contains an intrinsically disordered region and a Zα domain. Genome editing of CyHV-3 indicated that the expression of only the Zα domain of ORF112 was sufficient for normal viral replication in cell culture and virulence in carp. In contrast, its deletion was lethal for the virus. These observations revealed the potential of the CyHV-3 model as a unique platform to compare the exchangeability of Zα domains expressed alone in living cells. Attempts to rescue the ORF112 deletion by a broad spectrum of cellular, viral, and artificial Zα domains showed that only those expressing Z-binding activity, the capacity to induce liquid-liquid phase separation (LLPS), and A-to-Z conversion, could rescue viral replication. For the first time, this study reports the ability of some Zα domains to induce LLPS and supports the biological relevance of dsRNA A-to-Z conversion mediated by Zα domains. This study expands the functional diversity of Zα domains and stimulates new hypotheses concerning the mechanisms of action of proteins containing Zα domains.</jats:p>

収録刊行物

参考文献 (80)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ