<i>Toxoplasma gondii</i> profilin does not stimulate an innate immune response through bovine or human TLR5
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- Kata Tombácz
- Department of Pathology and Pathogen Biology, The Royal Veterinary College, University of London, UK
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- Gregg Burgess
- Department of Pathology and Pathogen Biology, The Royal Veterinary College, University of London, UK
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- Angela Holder
- Department of Pathology and Pathogen Biology, The Royal Veterinary College, University of London, UK
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- Arno Werners
- Department of Anatomy, Physiology and Pharmacology, School of Veterinary Medicine, St. George’s University, Grenada, West Indies
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- Dirk Werling
- Department of Pathology and Pathogen Biology, The Royal Veterinary College, University of London, UK
説明
<jats:p> Toxoplasma gondii is responsible for one of the most prevalent infections in people. T. gondii profilin (TgPr) is a protein integral to parasite movement and cellular invasion. Murine TLRs has been described to bind TgPr. Furthermore, more recently, human TLR5 has been described to recognise recombinant TgPr, as well as bacterial flagellin. In addition to infections in humans, T. gondii infects farm animals, but little information is available about its innate recognition. We aimed to investigate whether, similarly to their human orthologue, bovine and porcine TLR5 could also be stimulated by TgPr by using a combination of reporter cell lines expressing full length TLR5 from each species as well as primary cells. Although human and bovine TLR5-transfected cells responded to flagellin, no response was detected upon stimulation with profilin. Furthermore, TgPr failed to elicit IL-6 secretion in human peripheral blood mononuclear cells and CD14<jats:sup>+</jats:sup> monocytes. In contrast, exposure of RAW cells, known to express TLR11, to TgPr slightly increased the IL-6 response. Our data cast doubts on the possibility that profilin is a specific ligand for human TLR5 and bovine TLR5. This leaves the immunogenic properties of this potential target antigen (Ag) uncharacterised outside of the murine system. </jats:p>
収録刊行物
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- Innate Immunity
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Innate Immunity 24 (7), 422-429, 2018-09-08
SAGE Publications
