Branched Antisense and siRNA Co‐Assembled Nanoplatform for Combined Gene Silencing and Tumor Therapy
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- Jianbing Liu
- CAS Key Laboratory of Nanosystem and Hierarchical Fabrication CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology 11 BeiYiTiao, ZhongGuanCun Beijing 100190 China
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- Xuehe Lu
- CAS Key Laboratory of Nanosystem and Hierarchical Fabrication CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology 11 BeiYiTiao, ZhongGuanCun Beijing 100190 China
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- Tiantian Wu
- CAS Key Laboratory of Nanosystem and Hierarchical Fabrication CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology 11 BeiYiTiao, ZhongGuanCun Beijing 100190 China
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- Xiaohui Wu
- CAS Key Laboratory of Nanosystem and Hierarchical Fabrication CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology 11 BeiYiTiao, ZhongGuanCun Beijing 100190 China
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- Lin Han
- CAS Key Laboratory of Nanosystem and Hierarchical Fabrication CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology 11 BeiYiTiao, ZhongGuanCun Beijing 100190 China
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- Baoquan Ding
- CAS Key Laboratory of Nanosystem and Hierarchical Fabrication CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology 11 BeiYiTiao, ZhongGuanCun Beijing 100190 China
説明
<jats:title>Abstract</jats:title><jats:p>Chemically modified DNA has been widely developed to fabricate various nucleic acid nanostructures for biomedical applications. Herein, we report a facile strategy for construction of branched antisense DNA and small interfering RNA (siRNA) co‐assembled nanoplatform for combined gene silencing in vitro and in vivo. In our design, the branched antisense can efficiently capture siRNA with 3′ overhangs through DNA–RNA hybridization. After being equipped with an active targeting group and an endosomal escape peptide by host–guest interaction, the tailored nucleic acid nanostructure functions efficiently as both delivery carrier and therapeutic cargo, which is released by endogenous RNase H digestion. The multifunctional nucleic acid nanosystem elicits an efficient inhibition of tumor growth based on the combined gene silencing of the tumor‐associated gene polo‐like kinase 1 (PLK1). This biocompatible nucleic acid nanoplatform presents a new strategy for the development of gene therapy.</jats:p>
収録刊行物
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- Angewandte Chemie International Edition
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Angewandte Chemie International Edition 60 (4), 1853-1860, 2020-11-24
Wiley
