Baculovirus-Expressed Constructs Induce Immunoglobulin G That Recognizes VAR2CSA on<i>Plasmodium falciparum</i>- Infected Erythrocytes

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  • Lea Barfod
    Centre for Medical Parasitology at Copenhagen University Hospital (Rigshospitalet) and Institute for Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark
  • Morten A. Nielsen
    Centre for Medical Parasitology at Copenhagen University Hospital (Rigshospitalet) and Institute for Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark
  • Louise Turner
    Centre for Medical Parasitology at Copenhagen University Hospital (Rigshospitalet) and Institute for Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark
  • Madeleine Dahlbäck
    Centre for Medical Parasitology at Copenhagen University Hospital (Rigshospitalet) and Institute for Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark
  • Anja T. R. Jensen
    Centre for Medical Parasitology at Copenhagen University Hospital (Rigshospitalet) and Institute for Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark
  • Lars Hviid
    Centre for Medical Parasitology at Copenhagen University Hospital (Rigshospitalet) and Institute for Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark
  • Thor G. Theander
    Centre for Medical Parasitology at Copenhagen University Hospital (Rigshospitalet) and Institute for Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark
  • Ali Salanti
    Centre for Medical Parasitology at Copenhagen University Hospital (Rigshospitalet) and Institute for Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark

Description

<jats:title>ABSTRACT</jats:title><jats:p>We raised specific antisera against recombinant VAR2CSA domains produced in<jats:italic>Escherichia coli</jats:italic>and in insect cells. All were reactive in enzyme-linked immunosorbent assay, but only insect cell-derived constructs induced immunoglobulin G (IgG) that was reactive with native VAR2CSA on the surface of infected erythrocytes. Our data show that five of the six VAR2CSA Duffy-binding-like domains are surface exposed and that induction of surface-reactive VAR2CSA-specific IgG depends critically upon antigen conformation. These findings have implications for the development of vaccines against pregnancy-associated<jats:italic>Plasmodium falciparum</jats:italic>malaria.</jats:p>

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