Investigations of oocyte <i>in vitro</i> maturation within a mouse model

DOI Web Site 被引用文献1件

説明

<jats:p>This study attempted to develop a ‘less meiotically competent’ murine model for oocyte <jats:italic>in vitro</jats:italic> maturation (IVM), which could more readily be extrapolated to human clinical assisted reproduction. Oocyte meiotic competence was drastically reduced upon shortening the standard duration of <jats:italic>in vivo</jats:italic> gonadotrophin stimulation from 48 h to 24 h, and by selecting only naked or partially naked germinal vesicle oocytes, instead of fully cumulus enclosed oocyte complexes. With such a less meiotically competent model, only porcine granulosa coculture significantly enhanced the oocyte maturation rate <jats:italic>in vitro</jats:italic>, whereas no significant enhancement was observed with macaque and murine granulosa coculture. Increased serum concentrations and the supplementation of gonadotrophins, follicular fluid and extracellular matrix gel within the culture medium did not enhance IVM under either cell-free or coculture conditions. Culture medium conditioned by porcine granulosa also enhanced the maturation rate, and this beneficial effect was not diminished upon freeze–thawing. Enhanced IVM in the presence of porcine granulosa coculture did not, however, translate into improved developmental competence, as assessed by <jats:italic>in vitro</jats:italic> fertilization and embryo culture to the blastocyst stage.</jats:p>

収録刊行物

  • Zygote

    Zygote 12 (1), 1-18, 2004-02

    Cambridge University Press (CUP)

被引用文献 (1)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ