Engineering of human brain organoids with a functional vascular-like system
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- Bilal Cakir
- Yale University
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- Yangfei Xiang
- Yale University
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- In-Hyun Park
- Yale University
書誌事項
- 公開日
- 2019-10-07
- 資源種別
- preprint
- 権利情報
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- https://creativecommons.org/licenses/by/4.0/
- DOI
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- 10.21203/rs.2.13464/v1
- 公開者
- Springer Science and Business Media LLC
説明
<title>Abstract</title> <p>Here, we engineered human embryonic stem cells (hESCs) to ectopically express human ETS variant 2 (ETV2) to create in vitro vasculature in cortical organoids (hCOs), namely vhCOs (vascularized hCOs). ETV2-expressing cells in hCOs contributed to forming a complex vascular-like network in hCOs. This protocol describes hESC maintenance, the generation of inducible ETV2 expressing hESCs, and the generation of cortical organoid with functional vascular-like network.</p>